Expression and roles of Slit/Robo in human ovarian cancer.
Expression and roles of Slit/Robo in human ovarian cancer.
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DOI：
10.1007/s00418-011-0806-2
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发表时间：
2011-05
影响因子：
2.3
通讯作者：
Zheng, Jing
中科院分区：
文献类型：
--
作者：
Dai, Cai Feng;Jiang, Yi Zhou;Li, Yan;Wang, Kai;Liu, Pei Shu;Patankar, Manish S.;Zheng, Jing
狭缝糖蛋白及其环形（Robo）受体调节包括人类癌细胞在内的多种细胞的迁移和生长。然而，人们对Slit/Robo在人卵巢癌中的表达和作用知之甚少。在此，我们利用两种卵巢癌细胞系OVCAR-3和SKOV-3检测了Slit/Robo在人正常和恶性卵巢组织中的表达，以及它在调节人卵巢癌细胞迁移和增殖中的潜在作用。我们证明了Slit2/3和Robo1主要在人类正常卵巢的基质细胞和卵巢癌组织的许多组织类型的癌细胞中免疫定位。在OVCAR-3和SKOV-3细胞中也发现了Slit2/3和Robo1/4蛋白的表达。而重组人Slit2对SKOV-3细胞的迁移以及OVCAR-3和SKOV-3细胞的增殖没有显著影响。Slit2在OVCAR-3和SKOV-3细胞中也没有诱导ERK1/2和AKT1磷酸化。目前的研究结果表明，Slit/Robo家族的三个主要成员（Slit2/3和Robo1）广泛表达于人类正常和恶性卵巢组织以及OVCAR-3和SKOV-3细胞中。然而，Slit/Robo信号可能在调节人卵巢癌细胞增殖和迁移中不起重要作用。
The Slit glycoproteins and their Roundabout (Robo) receptors regulate migration and growth of many types of cells including human cancer cells. However, little is known about the expression and roles of Slit/Robo in human ovarian cancer. Herein, we examined the expression of Slit/Robo in human normal and malignant ovarian tissues and its potential participation in regulating migration and proliferation of human ovarian cancer cells using two ovarian cancer cell lines, OVCAR-3 and SKOV-3. We demonstrated that Slit2/3 and Robo1 were immunolocalized primarily in stromal cells in human normal ovaries and in cancer cells in many histotypes of ovarian cancer tissues. Protein expression of Slit2/3 and Robo1/4 was also identified in OVCAR-3 and SKOV-3 cells. However, recombinant human Slit2 did not significantly affect SKOV-3 cell migration, and OVCAR-3 and SKOV-3 cell proliferation. Slit2 also did not induce ERK1/2 and AKT1 phosphorylation in OVCAR-3 and SKOV-3 cells. The current findings indicate that three major members (Slit2/3 and Robo1) of Slit/Robo family are widely expressed in the human normal and malignant ovarian tissues and in OVCAR-3 and SKOV-3 cells. However, Slit/Robo signaling may not play an important role in regulating human ovarian cancer cell proliferation and migration.
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影响因子：
64.5
作者：
Brose, K;Bland, KS;Kidd, T
通讯作者：
Kidd, T
胎盘血管生成中缝隙/机器人信号传导的观点。
影响因子：
2
作者：
Liao WX;Wing DA;Geng JG;Chen DB
通讯作者：
Chen DB
DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
8
作者：
Dallol, A;Forgacs, E;Latif, F
通讯作者：
Latif, F
影响因子：
2.6
作者：
Liu, JM;Zhang, L;Hu, HY
通讯作者：
Hu, HY