Deleted HTLV-I provirus in blood and cutaneous lesions of patients with mycosis fungoides.
Deleted HTLV-I provirus in blood and cutaneous lesions of patients with mycosis fungoides.
复制标题
删除蕈样肉芽肿患者血液和皮肤病变中的 HTLV-I 原病毒。
作者：
W. Hall;Chunren R. Liu;O. Schneewind;Hidehiro Takahashi;M. Kaplan;G. Roupe;A. Vahlne
蕈样肉芽肿是一种罕见的皮肤T细胞白血病/淋巴瘤，基于与成人T细胞白血病的某些相似性，怀疑其具有病毒病因，成人T细胞白血病与人类T细胞白血病/淋巴瘤病毒I型（HTLV-I）感染有关。从患有蕈样肉芽肿的HTLV-I血清阴性患者的外周血单核细胞（PBMC）建立细胞系。DNA杂交分析显示，存在HTLV-I相关序列与不寻常的限制性内切酶位点。亚克隆片段的序列分析表明，存在一个单克隆整合的前病毒与5.5-丝氨酸蛋白酶缺失，涉及大区域的gag和env和所有的pol. Additional证据删除前病毒的存在被发现通过聚合酶链反应（PCR）扩增的DNA从皮肤病变的其他5个HTLV-I-血清阴性患者。研究结果表明，HTLV-I感染可能参与了至少某些蕈样肉芽肿病例的病因。
Mycosis fungoides, a rare form of cutaneous T cell leukemia/lymphoma, is suspected of having a viral etiology on the basis of certain similarities to adult T cell leukemia, which is associated with human T cell leukemia/lymphoma virus type I (HTLV-I) infection. Cell lines were established from peripheral blood mononuclear cells (PBMC) of an HTLV-I-seronegative patient with mycosis fungoides. DNA hybridization analysis revealed the presence of HTLV-I-related sequences with unusual restriction endonuclease sites. Sequence analysis of subcloned fragments demonstrated the presence of a monoclonally integrated provirus with a 5.5-kilobase deletion involving large regions of gag and env and all of pol. Additional evidence for the presence of deleted proviruses was found by polymerase chain reaction (PCR) amplification of DNA from cutaneous lesions of five other HTLV-I-seronegative patients. The findings suggest that HTLV-I infection may be involved in the etiology of at least certain cases of mycosis fungoides.
影响因子：
56.9
作者：
HAROUSE, JM;BHAT, S;GONZALEZSCARANO, F
通讯作者：
GONZALEZSCARANO, F
影响因子：
56.9
作者：
SLAMON, DJ;SHIMOTOHNO, K;CHEN, ISY
通讯作者：
CHEN, ISY