Sexual differences in the sialomes of the zebra tick, Rhipicephalus pulchellus.
Sexual differences in the sialomes of the zebra tick, Rhipicephalus pulchellus.
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DOI：
10.1016/j.jprot.2014.12.014
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发表时间：
2015-03-18
影响因子：
3.3
通讯作者：
Ribeiro JM
中科院分区：
文献类型：
--
作者：
Tan AW;Francischetti IM;Slovak M;Kini RM;Ribeiro JM
蜱虫完全依靠脊椎动物的血液生存。在进食过程中，蜱虫会向宿主体内注射一种复杂的唾液药水，以克服宿主的止血和不良炎症反应。这些介质也可以增强病原体的传播。蜱唾液蛋白库的知识可能会导致疫苗的目标，破坏喂养和/或寄生虫传播，以及发现新的药理学试剂。男性的唾液也可能有助于繁殖，因为男性使用他们的口器润滑并将他们的精子囊引入女性的生殖孔。对雄蜱和雌蜱唾液腺的分析使我们能够独立地了解每种性别成功进食的策略。使用Illumina HiSeq方案，在不同时间点饲喂pulchellus。总共241，229，128个配对末端读段的从头组装导致提取50，460个编码序列（CDS），其中11，277个对已知转录物具有超过75%的覆盖率，或代表新序列，并提交给GenBank。此外，我们从雄性和雌性的唾液腺提取物中产生了蛋白质组。pulchellus，分别产生总共454和2，063个蛋白质，其通过一个或多个肽以至少95%的置信度鉴定。数据集以带注释的超链接Excel电子表格形式呈现，描述了121个推定的分泌蛋白家族。确定了女性和男性特异性转录物。
Ticks rely exclusively on vertebrate blood for their survival. During feeding ticks inject into their hosts a sophisticated salivary potion that overcomes host hemostasis and adverse inflammatory responses. These mediators may also enhance pathogen transmission. Knowledge of the tick salivary protein repertoire may lead to vaccine targets to disrupt feeding and/or parasite transmission as well as to the discovery of novel pharmacological agents. Male saliva may also assist reproduction because males use their mouthparts to lubricate and introduce their spermatophores into the females’ genital pore. The analyses of the sialomes of male and female ticks independently allow us to understand the strategy used by each gender to feed successfully.We sequenced cDNA libraries from pools of salivary glands from adult male and female R. pulchellus feeding at different time points, using the Illumina HiSeq protocol. De novo assembly of a total of 241,229,128 paired-end reads lead to extraction of 50,460 coding sequences (CDS), 11,277 of which had more than 75% coverage to known transcripts, or represented novel sequences, and were submitted to GenBank. Additionally, we generated the proteome, from the salivary gland extracts of male and female R. pulchellus, yielding a total of 454 and 2,063 proteins respectively which were identified by one or more peptides with at least 95% confidence. The data set is presented as an annotated hyperlinked Excel spreadsheet, describing 121 putative secreted protein families. Female and male specific transcripts were identified.
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影响因子：
2.2
作者：
FELDMANM.B;BORUT, S;SALITERN.
通讯作者：
SALITERN.
影响因子：
4.1
作者：
Calvete, JJ;Moreno-Murciano, MP;Marcinkiewicz, C
通讯作者：
Marcinkiewicz, C
DOI：
10.1111/j.1538-7836.2009.03553.x
发表时间：
2009-11
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Carneiro-Lobo TC;Konig S;Machado DE;Nasciutti LE;Forni MF;Francischetti IM;Sogayar MC;Monteiro RQ
通讯作者：
Monteiro RQ
影响因子：
3.6
作者：
Blandin, S;Levashina, EA
通讯作者：
Levashina, EA
影响因子：
4.8
作者：
Arolas, JL;Lorenzo, J;Sommerhoff, CP
通讯作者：
Sommerhoff, CP