Validation of a 1DL earliness per se (eps) flowering QTL in bread wheat (Triticum aestivum).
Validation of a 1DL earliness per se (eps) flowering QTL in bread wheat (Triticum aestivum).
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DOI：
10.1007/s11032-014-0094-3
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Griffiths S
中科院分区：
文献类型：
--
作者：
Zikhali M;Leverington-Waite M;Fish L;Simmonds J;Orford S;Wingen LU;Goram R;Gosman N;Bentley A;Griffiths S
春化、光周期和相对不明确的提前本身（eps）基因调节植物的开花。我们在此报告使用近等基因系 (NIL) 对位于小麦 1DL 上的主要 eps 数量性状基因座 (QTL) 进行的验证。我们使用了来自 Spark 和 Rialto 冬小麦品种杂交的四对独立的 NIL，这些冬小麦品种在田间和受控环境中生长。携带 Spark 等位基因（由 QTL 侧翼标记 Xgdm111 和 Xbarc62 定义）的 NIL 在完全春化时始终比带有 Rialto 的 NIL 提前 3-5 天开花。在田间条件、短日照（10 小时光照）、长日照（16 小时光照）和超长日照（20 小时光照）下，该效应与光周期无关。这些结果验证了我们使用双单倍体 (DH) 群体鉴定的原始 QTL。该 QTL 代表了西北欧优质冬小麦种质中维持的变异。用于开发 NIL 的两个 DH 系 SR9 和 SR23 使我们能够定义标记 Xgdm111 下游 1DL QTL 的位置。 SR9 具有 Spark 1DL 臂，而 SR23 具有重组 1DL 臂，其带有从 Xgdm111 到远端的 Spark 等位基因。我们的工作表明，即使在克隆基因之前，标记辅助选择 eps 效应也是可行且有用的。这意味着 eps 基因可以按照与光周期和春化基因相同的方式进行定义和定位克隆。这项验证研究是精细定位并最终在六倍体小麦中直接克隆该基因的第一步。本文的在线版本 (doi:10.1007/s11032-014-0094-3) 包含补充材料，可供授权用户使用。
Vernalization, photoperiod and the relatively poorly defined earliness per se (eps) genes regulate flowering in plants. We report here the validation of a major eps quantitative trait locus (QTL) located on wheat 1DL using near isogenic lines (NILs). We used four independent pairs of NILs derived from a cross between Spark and Rialto winter wheat varieties, grown in both the field and controlled environments. NILs carrying the Spark allele, defined by QTL flanking markers Xgdm111 and Xbarc62, consistently flowered 3–5 days earlier when fully vernalized relative to those with the Rialto. The effect was independent of photoperiod under field conditions, short days (10-h light), long days (16-h light) and very long days (20-h light). These results validate our original QTL identified using doubled haploid (DH) populations. This QTL represents variation maintained in elite north-western European winter wheat germplasm. The two DH lines used to develop the NILs, SR9 and SR23 enabled us to define the location of the 1DL QTL downstream of marker Xgdm111. SR9 has the Spark 1DL arm while SR23 has a recombinant 1DL arm with the Spark allele from Xgdm111 to the distal end. Our work suggests that marker assisted selection of eps effects is feasible and useful even before the genes are cloned. This means eps genes can be defined and positionally cloned in the same way as the photoperiod and vernalization genes have been. This validation study is a first step towards fine mapping and eventually cloning the gene directly in hexaploid wheat. The online version of this article (doi:10.1007/s11032-014-0094-3) contains supplementary material, which is available to authorized users.
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