Engineering light-initiated afterglow lateral flow immunoassay for infectious disease diagnostics.
Engineering light-initiated afterglow lateral flow immunoassay for infectious disease diagnostics.
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用于传染病诊断的光引发余辉侧流免疫分析
DOI：
10.1016/j.bios.2022.114411
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发表时间：
2022-09-15
影响因子：
12.6
通讯作者：
中科院分区：
文献类型：
--
作者：
高传染性疾病的大流行对床旁检测（POCT）的高灵敏度和适应能力提出了迫切要求。在本文中，我们首次报道了一种聚集诱导发射（AIE）染料激发光引发余辉纳米探针（命名为LiAGNPs），实施到侧流免疫测定（LFIA）测试条上，用于诊断两种高度传染性疾病，人类免疫缺陷病毒（HIV）和严重急性呼吸综合征冠状病毒2（SARS-CoV-2）作为模型验证。主要工作机制依赖于周期性产生的单线态氧（1 O2）触发的LiAGNPs的时间分辨发光信号，其中负载AIE染料（TTMN）和荧光底物（SO）。所设计的LiAGNPs被发现比目前使用的基于Eu（III）的时间分辨荧光纳米颗粒和金纳米颗粒在侧流免疫测定（LFIA）中分别灵敏2倍和32倍。此外，LiAGNPs的裸色和荧光的额外光学行为使具有裸眼和荧光检测能力的LFIA平台能够满足各种场景下的应用。简而言之，多功能LiAGNPs作为一种新型的时间分辨报告基因，在提高检测灵敏度和应用灵活性方面具有巨大的潜力，与LFIA平台一起用于快速但敏感的传染病诊断。在这项工作中，我们报告了一种新的光引发的余辉纳米报告具有显着的时间分辨发光功能，并证明其潜在的敏感检测的传染病的测试条。
The pandemic of highly contagious diseases has put forward urgent requirements for high sensitivity and adaptive capacity of point-of-care testing (POCT). Herein, for the first time, we report an aggregation-induced emission (AIE) dye-energized light-initiated afterglow nanoprobes (named LiAGNPs), implemented onto a lateral flow immunoassay (LFIA) test strip, for diagnosis of two highly contagious diseases, human immunodeficiency virus (HIV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as model validation. The primary working mechanism relies on the cyclically generated singlet oxygen (1O2)-triggered time-resolved luminescent signals of LiAGNPs in which AIE dyes (TTMN) and chemiluminescent substrates (SO) are loaded. The designed LiAGNPs were found 2-fold and 32-fold sensitive than the currently used Eu(III)-based time-resolved fluorescent nanoparticles and gold nanoparticles in lateral flow immunoassay (LFIA), respectively. In addition, the extra optical behaviors of nude color and fluorescence of LiAGNPs enable the LFIA platform with the capability of the naked eye and fluorescent detection to satisfy the applications under varying scenarios. In short, the versatile LiAGNPs have great potential as a novel time-resolved reporter in enhancing detection sensitivity and application flexibility with LFIA platform for rapid but sensitive infectious disease diagnostics. In this work, we report a novel light-Initiated afterglow nanoreporter with a remarkable time-resolved luminescence feature and testify its potential for sensitive detection of infectious disease on test strip.
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DOI：
10.1073/pnas.2014739117
发表时间：
2020-09-15
影响因子：
11.1
作者：
Ganguli A;Mostafa A;Berger J;Aydin MY;Sun F;Ramirez SAS;Valera E;Cunningham BT;King WP;Bashir R
通讯作者：
Bashir R
用于具有灵活双模态切换的高灵敏度侧流免疫测定的分层等离子体荧光标记
影响因子：
9.5
作者：
Huang, Liang;Jin, Jiening;Hu, Jun
通讯作者：
Hu, Jun
用于快速检测 SARS-CoV-2 和甲型 H1N1 流感病毒的多通道免疫传感器平台
影响因子：
9.5
作者：
Li, Jianyong;Lin, Rui;Hao, Rongzhang
通讯作者：
Hao, Rongzhang
影响因子：
16.6
作者：
Chen, Jing;Hao, Liangwen;Tang, Ben Zhong
通讯作者：
Tang, Ben Zhong
影响因子：
64.8
作者：
Ackerman, Cheri M.;Myhrvold, Cameron;Sabeti, Pardis C.
通讯作者：
Sabeti, Pardis C.