Alcanivorax borkumensis Biofilms Enhance Oil Degradation By Interfacial Tubulation

Alcanivorax borkumensis Biofilms Enhance Oil Degradation By Interfacial Tubulation
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Alcanivorax borkumensis 生物膜通过界面管增强石油降解

DOI:
10.1101/2022.08.06.503017
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发表时间:
2022
期刊:
bioRxiv
影响因子:
--
通讯作者:
A. S. Utada*
A. S. Utada*
中科院分区:
--
文献类型:
--
作者:
M. Prasad;N. Obana;S.-Z. Lin;S. Zhao;K. Sakai;C. Blanch-Mercader;J. Prost;N. Nomura;J.-F. Rupprecht*;J. Fattaccioli*;A. S. Utada*

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在消耗烷烃的过程中,Alcanivorax borkumensis 会在油滴周围形成生物膜,但其在降解过程中所起的作用仍不清楚。我们发现生物膜形态发生了变化,这取决于对石油消耗的适应:较长的暴露时间会导致出现针对通过界面管状影响的石油消耗进行优化的树枝状生物膜。原位微流体跟踪使我们能够将管状结构与界面细胞排序中的局部缺陷相关联。我们证明了通过使用限制来定位缺陷来控制液滴变形,从而在液滴中引起凹痕。我们开发了一个模型来阐明生物膜形态,将管状结构与界面张力降低和细胞疏水性增加联系起来。
During the consumption of alkanes,Alcanivorax borkumensiswill form a biofilm around an oil droplet, but the role this plays during degradation remains unclear. We identified a shift in biofilm morphology that depends on adaptation to oil consumption: Longer exposure leads to the appearance of dendritic biofilms optimized for oil consumption effected through tubulation of the interface. In situ microfluidic tracking enabled us to correlate tubulation to localized defects in the interfacial cell ordering. We demonstrate control over droplet deformation by using confinement to position defects, inducing dimpling in the droplets. We developed a model that elucidates biofilm morphology, linking tubulation to decreased interfacial tension and increased cell hydrophobicity.
碳氢化合物生物降解过程中生物膜形成引起的限制现象
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