Short-term effects of calcium ions on the apoptosis and onset of mineralization of human dental pulp cells in vitro and in vivo.
Short-term effects of calcium ions on the apoptosis and onset of mineralization of human dental pulp cells in vitro and in vivo.
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DOI：
10.3892/ijmm.2015.2218
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发表时间：
2015-07
影响因子：
5.4
通讯作者：
Ling J
中科院分区：
文献类型：
--
作者：
An S;Gao Y;Huang Y;Jiang X;Ma K;Ling J
钙离子（Ca 2+）是大多数盖髓材料的主要成分，并且在人牙髓细胞（hDPC）的矿化中具有重要作用。我们小组先前的一项研究表明，在长期培养（21天）中，Ca 2+水平的增加可以促进hDPC介导的矿化。然而，矿化的起始发生在成骨诱导培养的早期阶段，并且在短期培养（5天）中Ca 2+对hDPC矿化的影响尚未详细研究。此外，Ca 2+刺激hDPC矿化的潜在机制仍然存在争议。矿化和细胞凋亡和/或死亡之间的强相关性已被确定。因此，本研究假设，Ca 2+可能通过诱导其凋亡和/或死亡来促进hDPC介导的矿化的发生。为了验证这一假设，将不同浓度的Ca 2+加入到生长培养基和成骨培养基中。茜素红S染色和逆转录聚合酶链反应分析被用来评估矿化的开始。此外，采用细胞计数试剂盒-8和异硫氰酸荧光素-膜联蛋白V/碘化丙啶双染色法检测生长培养基中hDPC的增殖和凋亡。通过动物实验和扫描电镜观察，对陶瓷移植种植体的体内矿化情况进行了研究。结果表明，5.4和9.0 mM Ca ~（2+）可促进矿化基质结节的形成，促进骨桥蛋白mRNA的表达，诱导细胞凋亡和坏死，但对细胞增殖无明显影响。这些结果表明，细胞凋亡和/或死亡和形成的时间，以及在短期培养的hDPC的钙诱导的细胞外矿化的量之间的正相关。
Calcium ions (Ca2+) are a major constituent of most pulp-capping materials and have an important role in the mineralization of human dental pulp cells (hDPCs). A previous study by our group has shown that increased levels of Ca2+ can promote hDPC-mediated mineralization in long-term cultures (21 days). However, the initiation of mineralization occurs in the early stage of osteogenic inductive culture, and the effects of Ca2+ on the mineralization of hDPCs in short-term cultures (five days) have not been studied in detail. Furthermore, the underlying mechanism by which Ca2+ stimulates the mineralization of hDPCs has remained controversial. A strong correlation between mineralization and cell apoptosis and/or death has been identified. Thus, the present study hypothesized that Ca2+ may promote the onset of hDPC-mediated mineralization through inducing their apoptosis and/or death. To verify this hypothesis, Ca2+ was added to the growth culture medium and osteogenic culture medium at various concentrations. Alizarin Red S staining and reverse transcription-polymerase chain reaction analysis were used to evaluate the onset of mineralization. Furthermore, the cell counting kit-8 and fluorescein isothiocyanate-Annexin V/propidium iodide double-staining method were adopted to detect the proliferation and apoptosis of hDPCs in the growth culture medium. An animal experiment and scanning electron microscopic observation of ceramic graft implants were applied to measure the mineralization in vivo. The results showed that 5.4 and 9.0 mM Ca2+ accelerated the onset of mineralized matrix nodule formation, promoted osteopontin mRNA expression and induced marked cell apoptosis and necrosis, but had no obvious effect on cell proliferation. These findings indicated a positive association between cell apoptosis and/or death and the timing of formation as well as the quantity of extracellular mineralization induced by Ca2+ in short-term cultured hDPCs.
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影响因子：
2.2
作者：
Hilton TJ
通讯作者：
Hilton TJ
影响因子：
6.5
作者：
HIROTA, S;ASADA, H;KITAMURA, Y
通讯作者：
KITAMURA, Y
DOI：
10.1007/s10856-011-4531-0
发表时间：
2012-03-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.2
作者：
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通讯作者：
Kohri, Kenjiro