Genome evolution in major Escherichia coli O157:H7 lineages.
Genome evolution in major Escherichia coli O157:H7 lineages.
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DOI：
10.1186/1471-2164-8-121
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发表时间：
2007-05-16
期刊：
影响因子：
4.4
通讯作者：
Gannon VP
中科院分区：
文献类型：
--
作者：
Zhang Y;Laing C;Steele M;Ziebell K;Johnson R;Benson AK;Taboada E;Gannon VP
大肠杆菌O 157：H7菌株的遗传分析表明，分化成两个不同的谱系，谱系I和II，似乎具有不同的生态特征，谱系I菌株更常见于人类疾病。本研究利用基因芯片比较基因组杂交（CGH）技术，对31株大肠杆菌进行了基因组差异分析。coli O 157：H7菌株属于不同的噬菌体类型（PT）和不同的谱系特异性多态性测定（LSPA）类型。在所有E. coliO 157：H7菌株和1,751株存在或不存在。在此基础上，E.大肠杆菌O 157：H7菌株可分为3个不同的类群，分别由15个谱系I（LSPA 111111）、4个谱系I/II（本研究命名为LSPA 211111）和12个谱系II菌株（LSPA 22222、222211、222212和222221）组成。在谱系I菌株中占优势的11个不同的基因组区域（存在于≥80%的谱系I中，而不存在于≥ 92%的谱系II菌株中）跨越每个包含少至2个和多达25个ORF的片段。这些区域在E. coli Sakai S-环#14、16、69、72、78、83、85、153和286，Sakai噬菌体10（S-环#91、92和93）和基因组骨架区。所有四个谱系I/II株PT 2，并拥有这11个谱系I-显性位点中的8个。在I系和II系菌株之间注意到毒力相关位点的几个差异，包括编码滋贺毒素2的S环69内的分歧，以及II系菌株中不存在非LEE编码的效应基因nleF和nleH 1 -2以及perC同源基因pchD。CGH数据表明，在E. coli O157：H7。这些亚组的基因组组成支持已从其他方法推断出的同源性，并进一步表明，从祖先的形式和横向基因转移的基因组分歧，有助于他们的进化。在这项研究中确定的基因组特征可能有助于不同的大肠杆菌菌株在流行病学和生态学上的明显差异。coliO 157：H7谱系。
Genetic analysis of Escherichia coli O157:H7 strains has shown divergence into two distinct lineages, lineages I and II, that appear to have distinct ecological characteristics, with lineage I strains more commonly associated with human disease. In this study, microarray-based comparative genomic hybridization (CGH) was used to identify genomic differences among 31 E. coli O157:H7 strains that belong to various phage types (PTs) and different lineage-specific polymorphism assay (LSPA) types. A total of 4,084 out of 6,057 ORFs were detected in all E. coli O157:H7 strains and 1,751 were variably present or absent. Based on this data, E. coli O157:H7 strains were divided into three distinct clusters, which consisted of 15 lineage I (LSPA type 111111), four lineage I/II (designated in this study) (LSPA type 211111) and 12 lineage II strains (LSPA 222222, 222211, 222212, and 222221), respectively. Eleven different genomic regions that were dominant in lineage I strains (present in ≥80% of lineage I and absent from ≥ 92% of lineage II strains) spanned segments containing as few as two and up to 25 ORFs each. These regions were identified within E. coli Sakai S-loops # 14, 16, 69, 72, 78, 83, 85, 153 and 286, Sakai phage 10 (S-loops # 91, 92 and 93) and a genomic backbone region. All four lineage I/II strains were of PT 2 and possessed eight of these 11 lineage I-dominant loci. Several differences in virulence-associated loci were noted between lineage I and lineage II strains, including divergence within S-loop 69, which encodes Shiga toxin 2, and absence of the non-LEE encoded effector genes nleF and nleH1-2 and the perC homologue gene pchD in lineage II strains. CGH data suggest the existence of two dominant lineages as well as LSPA type and PT-related subgroups within E. coli O157:H7. The genomic composition of these subgroups supports the phylogeny that has been inferred from other methods and further suggests that genomic divergence from an ancestral form and lateral gene transfer have contributed to their evolution. The genomic features identified in this study may contribute to apparent differences in the epidemiology and ecology of strains of different E. coli O157:H7 lineages.
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影响因子：
4.2
作者：
Dowd SE;Ishizaki H
通讯作者：
Ishizaki H
影响因子：
9.4
作者：
Karmali, MA;Mascarenhas, M;Kaper, JB
通讯作者：
Kaper, JB
DOI：
10.1073/pnas.96.23.13288
发表时间：
1999-11-09
影响因子：
11.1
作者：
Kim, J;Nietfeldt, J;Benson, AK
通讯作者：
Benson, AK
影响因子：
2.8
作者：
Iyoda, S;Watanabe, H
通讯作者：
Watanabe, H
影响因子：
3.1
作者：
Batisson, I;Guimond, MP;Harel, J
通讯作者：
Harel, J