Modulation of human T-lymphocyte functions by the consumption of carotenoid-rich vegetables
Modulation of human T-lymphocyte functions by the consumption of carotenoid-rich vegetables
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食用富含类胡萝卜素的蔬菜对人类 T 淋巴细胞功能的调节
DOI：
10.1017/s0007114599001634
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发表时间：
1999
影响因子：
3.6
通讯作者：
G. Rechkemmer
中科院分区：
文献类型：
--
作者：
B. Watzl;A. Bub;Birgit R. Brandstetter;G. Rechkemmer
进行了一项人类干预研究，以确定食用富含类胡萝卜素的蔬菜对免疫系统的影响。不吸烟的受试者（23 名男性）的日常饮食不受限制，只是在整个研究期间必须戒除富含类胡萝卜素的水果和蔬菜。该研究分为四个阶段，每个阶段持续 2 周： 第 1-2 周：低类胡萝卜素阶段；第3-8周：每日饮用330毫升番茄汁（40毫克番茄红素/天，1·5毫克β-胡萝卜素/天）（第3-4周），330毫升胡萝卜汁（21·6毫克β-胡萝卜素/天，15·7毫克α-胡萝卜素/天，0·5毫克叶黄素/天）（第5-6周），10克干菠菜粉末（11·3 mg 叶黄素/天，3·1 mg β-胡萝卜素/天）（第 7-8 周）。禁食 12 小时后，每周从受试者身上采集血液。通过测量免疫反应性细胞因子的增殖和分泌来评估 T 淋巴细胞功能。摄入低类胡萝卜素饮食导致伴刀豆球蛋白 A 培养的外周血单核细胞 (PBMC) 的增殖显着降低。摄入番茄汁 2 周后直至干预期结束，与消耗期结束时的增殖相比，淋巴细胞增殖没有显着变化。辅助性T细胞1样淋巴细胞（白细胞介素（IL）-2）和辅助性T细胞2样淋巴细胞（IL-4）分泌的细胞因子受到饮食干预的影响。低类胡萝卜素饮食后，IL-2和IL-4分泌值显着受到抑制（与基线相比分别P<0·001和P<0·05）。与消耗期末相比，番茄汁消耗显着增强了IL-2 (P < 0·001) 和IL-4 的分泌(P < 0·05)。食用胡萝卜汁和菠菜粉后，PBMC 的细胞因子分泌能力与消耗期结束时没有显着差异。总之，本研究的结果表明，低类胡萝卜素饮食会降低 T 淋巴细胞功能，而添加番茄汁可以恢复这些功能。这种调节不能用血浆类胡萝卜素浓度的变化来解释。番茄汁中的活性成分以及这种免疫调节的生物学意义仍有待确定。
A human intervention study was conducted to determine the effect of the consumption of carotenoid-rich vegetables on the immune system. Subjects, (twenty-three men), who were non-smokers, were not restricted in their daily diet, except that they had to abstain from fruit and vegetables high in carotenoids throughout the whole study period. The study was divided into four periods, each lasting 2 weeks: weeks 1–2: low-carotenoid period; throughout weeks 3–8: daily consumption of 330 ml tomato juice (40 mg lycopene/d, 1·5 mg β-carotene/d) (weeks 3–4), 330 ml carrot juice (21·6 mg β-carotene/d, 15·7 mg α-carotene/d, 0·5 mg lutein/d) (weeks 5–6), 10 g dried spinach powder (11·3 mg lutein/d, 3·1 mg β-carotene/d) (weeks 7–8). Blood was collected weekly from subjects after a 12 h fast. T-lymphocyte functions were assessed by measuring proliferation and secretion of immunoreactive cytokines. The consumption of a low-carotenoid diet resulted in a significantly reduced proliferation of peripheral blood mononuclear cells (PBMC) cultured with concanavalin A. After 2 weeks of tomato juice consumption and until the end of the intervention period lymphocyte proliferation was not significantly changed compared with proliferation at the end of the depletion period. Secretion of cytokines by T-helper-1-like lymphocytes (interleukin (IL)-2) and by T-helper-2-like lymphocytes (IL-4) was influenced by the dietary intervention. IL-2 and IL-4 secretion values were significantly suppressed after the low-carotenoid diet (P <0·001 and P < 0·05 respectively compared with baseline). Tomato juice consumption significantly enhanced IL-2 (P < 0·001) and IL-4 secretion (P < 0·05) compared with the end of depletion period. After carrot juice and spinach powder consumption the cytokine secretion capacity of PBMC was not significantly different from that at the end of the depletion period. In conclusion, the results of the present study indicate that a low-carotenoid diet reduces T-lymphocyte functions and addition of tomato juice restores these functions. This modulation could not be explained by changes in the plasma carotenoid concentrations. The active constituents in tomato juice as well as the biological significance of this immunomodulation remain to be determined.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
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DOI：
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