Comparative expression profiles of microRNA in left and right atrial appendages from patients with rheumatic mitral valve disease exhibiting sinus rhythm or atrial fibrillation.
Comparative expression profiles of microRNA in left and right atrial appendages from patients with rheumatic mitral valve disease exhibiting sinus rhythm or atrial fibrillation.
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DOI：
10.1186/1479-5876-12-90
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发表时间：
2014-04-06
影响因子：
7.4
通讯作者：
Wu ZK
中科院分区：
文献类型：
--
作者：
Liu H;Qin H;Chen GX;Liang MY;Rong J;Yao JP;Wu ZK
在风湿性二尖瓣疾病（RMVD）患者的右心房（RA）和左心房（LA）中发现了与房颤（AF）相关的microRNA（miRNAs）。然而，大多数研究仅集中在RA上，并且AF相关miRNA在RA和LA之间的潜在差异仍然未知。本研究的目的是进行miRNA表达谱分析，以比较RMVD患者右心耳（RAA）和左心耳（LAA）中AF相关miRNA的潜在差异。在二尖瓣置换术期间，从18名RMVD患者（10名AF患者）中获得了RAA和LAA的样本组织。从这些组织中，使用人miRNA微阵列创建并分析miRNA表达谱。然后，使用qRT-PCR分析12个选定的miRNA来验证结果。最后，利用miRFocus数据库预测了10个验证过的miRNAs的潜在靶点，并分析了它们的功能和潜在通路。在RAA中，发现了65种AF相关的miRNAs，并且显著失调（即28种miRNAs上调，37种下调）。在LAA中，发现了42种AF相关的miRNAs，并且显著失调（即22种miRNAs上调，20种下调）。其中23个在RAA和LAA中均有表达，45个仅在RAA中表达，19个仅在LAA中表达。最后，通过qRT-PCR验证的10个AF相关miRNA在RAA和LAA中的分布相似; 3个在RAA和LAA中均发现，5个仅在RAA中发现，2个仅在LAA中发现。确定了潜在的miRNA靶点和分子途径。我们发现房颤相关的miRNAs在RMVD患者的RAA和LAA中有不同的分布。这可能反映了RA和LA之间AF的不同miRNA机制。这些发现可能为RMVD患者AF的潜在机制提供新的见解。
The atrial fibrillation (AF) associated microRNAs (miRNAs) were found in the right atrium (RA) and left atrium (LA) from patients with rheumatic mitral valve disease (RMVD). However, most studies only focus on the RA; and the potential differences of AF-associated miRNAs between the RA and LA are still unknown. The aim of this study was to perform miRNA expression profiles analysis to compare the potential differences of AF-associated miRNAs in the right atrial appendages (RAA) and left atrial appendages (LAA) from RMVD patients. Samples tissues from the RAA and LAA were obtained from 18 RMVD patients (10 with AF) during mitral valve replacement surgery. From these tissues, miRNA expression profiles were created and analyzed using a human miRNA microarray. Then, the results were validated using qRT-PCR analysis for 12 selected miRNAs. Finally, potential targets of 10 validated miRNAs were predicted and their functions and potential pathways were analyzed using the miRFocus database. In RAA, 65 AF-associated miRNAs were found and significantly dysregulated (i.e. 28 miRNAs were up-regulated and 37 were down-regulated). In LAA, 42 AF-associated miRNAs were found and significantly dysregulated (i.e. 22 miRNAs were up-regulated and 20 were down-regulated). Among these AF-associated miRNAs, 23 of them were found in both RAA and LAA, 45 of them were found only in RAA, and 19 of them were found only in LAA. Finally, 10 AF-associated miRNAs validated by qRT-PCR were similarly distributed in RAA and LAA; 3 were found in both RAA and LAA, 5 were found only in RAA, and 2 were found only in LAA. Potential miRNA targets and molecular pathways were identified. We have found the different distributions of AF-associated miRNAs in the RAA and LAA from RMVD patients. This may reflect different miRNA mechanisms in AF between the RA and LA. These findings may provide new insights into the underlying mechanisms of AF in RMVD patients.
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影响因子：
7.4
作者：
Qin H;Chen GX;Liang MY;Rong J;Yao JP;Liu H;Wu ZK
通讯作者：
Wu ZK
影响因子：
3.4
作者：
Huang, Wen-Jun;Zhou, Rui;Yang, Jin-Liang
通讯作者：
Yang, Jin-Liang
影响因子：
5.5
作者：
Girmatsion, Zenawit;Biliczki, Peter;Ehrlich, Joachim R.
通讯作者：
Ehrlich, Joachim R.
影响因子：
3.2
作者：
CHAPEAU, C;GUTKOWSKA, J;CANTIN, M
通讯作者：
CANTIN, M
影响因子：
37.8
作者：
Lu, Yanjie;Zhang, Ying;Yang, Baofeng
通讯作者：
Yang, Baofeng