Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
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铜绿假单胞菌 DspI 的结构和功能研究：对其在 DSF 生物合成中的作用的影响
DOI：
10.1038/s41598-018-22300-1
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发表时间：
2018-03-02
影响因子：
4.6
通讯作者：
Bao R
中科院分区：
文献类型：
--
作者：
Liu L;Li T;Cheng XJ;Peng CT;Li CC;He LH;Ju SM;Wang NY;Ye TH;Lian M;Xiao QJ;Song YJ;Zhu YB;Yu LT;Wang ZL;Bao R
DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P. aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P. aeruginosa.
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影响因子：
14.8
作者：
Hmelo LR;Borlee BR;Almblad H;Love ME;Randall TE;Tseng BS;Lin C;Irie Y;Storek KM;Yang JJ;Siehnel RJ;Howell PL;Singh PK;Tolker-Nielsen T;Parsek MR;Schweizer HP;Harrison JJ
通讯作者：
Harrison JJ
影响因子：
11
作者：
Boon, Calvin;Deng, Yinyue;Zhang, Lian-Hui
通讯作者：
Zhang, Lian-Hui
影响因子：
2.6
作者：
Huang, Tzu-Pi;Wong, Amy C. Lee
通讯作者：
Wong, Amy C. Lee
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
0.6
作者：
Basri, D. F.;Jaffar, N.;Raj, L. Santhana
通讯作者：
Raj, L. Santhana