Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs.
Transcriptome analysis of mRNA and miRNA in skeletal muscle indicates an important network for differential Residual Feed Intake in pigs.
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DOI：
10.1038/srep11953
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发表时间：
2015-07-07
影响因子：
4.6
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Jing L;Hou Y;Wu H;Miao Y;Li X;Cao J;Brameld JM;Parr T;Zhao S
饲料效率（FE）可以通过饲料转化率（FCR）或剩余采食量（RFI）来测量。本研究测定了236头去势纯种约克郡公猪的FE相关表型，并选取10头RFI高、低的极端个体进行转录组分析。我们使用RNA-seq分析来确定骨骼肌中基因和miRNA的差异表达。共鉴定出99个差异表达基因（q ≤ 0.05）。下调的基因主要涉及线粒体能量代谢，包括FABP 3、RCAN、PPARGC 1（PGC-1A）、HK 2和PRKAG 2。上调表达的基因主要涉及骨骼肌分化和增殖，包括IGF 2、PDE 7A、CEBPD、PIK 3R 1和MYH 6。此外，15个差异表达的miRNAs（|log 2光纤通道|≥ 1，总读段计数≥ 20，p ≤ 0.05）。其中，低RFI猪的miR-136、miR-30 e-5 p、miR-1、miR-208 b、miR-199 a、miR-101和miR-29 c表达上调，而miR-215、miR-365- 5 p、miR-486、miR-1271、miR-145、miR-99 b、miR-191和miR-10 b表达下调。我们得出结论，可能通过AMPK-PGC-1A途径降低线粒体能量代谢，通过IGF-1/2和TGF-β信号传导途径增加肌肉生长，是改善猪（以及可能的其他家畜）FE的潜在策略。这项研究提供了新的见解的分子机制，决定RFI和FE在猪。
Feed efficiency (FE) can be measured by feed conversion ratio (FCR) or residual feed intake (RFI). In this study, we measured the FE related phenotypes of 236 castrated purebred Yorkshire boars, and selected 10 extreme individuals with high and low RFI for transcriptome analysis. We used RNA-seq analyses to determine the differential expression of genes and miRNAs in skeletal muscle. There were 99 differentially expressed genes identified (q ≤ 0.05). The down-regulated genes were mainly involved in mitochondrial energy metabolism, including FABP3, RCAN, PPARGC1 (PGC-1A), HK2 and PRKAG2. The up-regulated genes were mainly involved in skeletal muscle differentiation and proliferation, including IGF2, PDE7A, CEBPD, PIK3R1 and MYH6. Moreover, 15 differentially expressed miRNAs (|log2FC| ≥ 1, total reads count ≥ 20, p ≤ 0.05) were identified. Among them, miR-136, miR-30e-5p, miR-1, miR-208b, miR-199a, miR-101 and miR-29c were up-regulated, while miR-215, miR-365-5p, miR-486, miR-1271, miR-145, miR-99b, miR-191 and miR-10b were down-regulated in low RFI pigs. We conclude that decreasing mitochondrial energy metabolism, possibly through AMPK - PGC-1A pathways, and increasing muscle growth, through IGF-1/2 and TGF-β signaling pathways, are potential strategies for the improvement of FE in pigs (and possibly other livestock). This study provides new insights into the molecular mechanisms that determine RFI and FE in pigs.
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影响因子：
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作者：
Bartz, M.;Szydlowski, M.;Switonski, M.
通讯作者：
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Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.