Radioluminescence microscopy: measuring the heterogeneous uptake of radiotracers in single living cells.
Radioluminescence microscopy: measuring the heterogeneous uptake of radiotracers in single living cells.
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DOI：
10.1371/journal.pone.0046285
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xing L
中科院分区：
文献类型：
--
作者：
Pratx G;Chen K;Sun C;Martin L;Carpenter CM;Olcott PD;Xing L
放射性示踪剂在研究体内和体外的分子过程中发挥着重要作用。然而，目前的方法仅限于测量大细胞群体中的平均放射性示踪剂摄取量，因此缺乏量化细胞间差异的能力。在这里，我们应用一种名为放射发光显微镜的新技术，在标准的荧光显微镜环境中显示单个活细胞中的放射性示踪剂摄取。在这项技术中，活细胞被稀疏地培养在薄的闪烁板上，并与放射性示踪剂孵化。用高灵敏度的显微镜测量贝塔衰变后产生的光。放射发光显微镜显示单细胞摄取[18F]氟代脱氧葡萄糖(FDG)具有很强的异质性，这与葡萄糖类似物的荧光成像一致。我们还验证了FDG在单细胞中的动态摄取遵循标准的双组织隔室模型。最后，我们用融合的PET/荧光报告基因转染细胞，发现FHBG(一种用于转基因表达的PET放射性示踪剂)的摄取与荧光蛋白的表达一致。总之，这些结果表明，放射发光显微镜可以以单细胞分辨率显示放射性示踪剂的摄取，这可能在放射性示踪剂的精确表征中找到用处。
Radiotracers play an important role in interrogating molecular processes both in vitro and in vivo. However, current methods are limited to measuring average radiotracer uptake in large cell populations and, as a result, lack the ability to quantify cell-to-cell variations. Here we apply a new technique, termed radioluminescence microscopy, to visualize radiotracer uptake in single living cells, in a standard fluorescence microscopy environment. In this technique, live cells are cultured sparsely on a thin scintillator plate and incubated with a radiotracer. Light produced following beta decay is measured using a highly sensitive microscope. Radioluminescence microscopy revealed strong heterogeneity in the uptake of [18F]fluoro-deoxyglucose (FDG) in single cells, which was found consistent with fluorescence imaging of a glucose analog. We also verified that dynamic uptake of FDG in single cells followed the standard two-tissue compartmental model. Last, we transfected cells with a fusion PET/fluorescence reporter gene and found that uptake of FHBG (a PET radiotracer for transgene expression) coincided with expression of the fluorescent protein. Together, these results indicate that radioluminescence microscopy can visualize radiotracer uptake with single-cell resolution, which may find a use in the precise characterization of radiotracers.
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影响因子：
3.5
作者：
Ott, R;MacDonald, J;Wells, K
通讯作者：
Wells, K
影响因子：
14.8
作者：
Yamada, Katsuya;Saito, Mikako;Inagaki, Nobuya
通讯作者：
Inagaki, Nobuya
DOI：
10.1002/cyto.990080509
发表时间：
1987-09-01
期刊：
CYTOMETRY
影响因子：
--
作者：
ENSLEY, JF;MACIOROWSKI, Z;CRISSMAN, J
通讯作者：
CRISSMAN, J
DOI：
10.1007/s10967-007-0625-z
发表时间：
2007-06-01
影响因子：
1.6
作者：
Kanno, S.;Rai, H.;Nakanishi, T. M.
通讯作者：
Nakanishi, T. M.
DOI：
10.2967/jnumed.108.054098
发表时间：
2009-11
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Jadvar H;Alavi A;Gambhir SS
通讯作者：
Gambhir SS