Strain-dependent urethral response.
Strain-dependent urethral response.
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DOI：
10.1002/nau.21158
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发表时间：
2011-11
影响因子：
2
通讯作者：
Vorp, David A.
中科院分区：
文献类型：
--
作者：
Haworth, Donna J.;Kitta, Takeya;Morelli, Brian;Chew, Douglas W.;Yoshimura, Naoki;de Groat, William C.;Vorp, David A.
Sprague-Dawley（SD）大鼠是一种远缘繁殖的多用途品系，已很好地用于下尿路研究。然而，为了测试用于压力性尿失禁等疾病的新细胞疗法，具有免疫耐受性的近亲繁殖大鼠品系，如刘易斯大鼠，可能更有用。本研究的目的是揭示刘易斯和SD大鼠之间下尿路梗阻机制的任何差异。在正常（对照）刘易斯和SD大鼠以及双侧阴部神经切断产生的压力性尿失禁模型中，评估（1）体外横纹肌和平滑肌对尿道机械和功能特性的贡献，以及（2）体内横纹括约肌对漏尿点压力（LPP）和反射性尿失禁机制的贡献。对照组（刘易斯大鼠）的LPP显著较低，骨骼肌的快缩显著较少，并且与对照组（SD大鼠）相比，对横纹括约肌的依赖性较低，这通过α-银环蛇毒素神经肌肉阻滞未能降低LPP来表明。神经切断显著降低SD大鼠的LPP，但在刘易斯大鼠中没有。虽然刘易斯尿道比SD大鼠含有更多的平滑肌，但其在体外的活性较低，如低的尿道基线压力和对苯丙氨酸缺乏反应所示。我们已经观察到SD和刘易斯尿道的功能和机械特性的明显差异，并且已经表明刘易斯大鼠可能不适合作为通过神经切断的SUI的慢性模型。
The Sprague–Dawley (SD) rat, an out-bred, all-purpose strain, has served well for lower urinary tract research. However, to test new cellular therapies for conditions such as stress urinary incontinence, an in-bred rat strain with immune tolerance, such as the Lewis rat, may be more useful. The objective of this study was to reveal any differences in lower urinary tract continence mechanisms between the Lewis and SD rat. The contribution of (1) the striated and smooth muscle to the mechanical and functional properties of the urethra in vitro, and (2) the striated sphincter to leak point pressure (LPP) and reflex continence mechanisms in vivo were assessed in normal (control) Lewis and SD rats and in a model of stress urinary incontinence produced by bilateral pudendal nerve transection. Control, Lewis rats had significantly lower LPP, significantly less fast-twitch skeletal muscle and relied less on the striated sphincter for continence than control, SD rats, as indicated by the failure of neuromuscular blockade with alpha-bungarotoxin to reduce LPP. Nerve transection significantly decreased LPP in the SD rat, but not in the Lewis rat. Although the Lewis urethra contained more smooth muscle than the SD rat, it was less active in vitro as indicated by a low urethral baseline pressure and lack of response to phenylephrine. We have observed distinct differences in functional and mechanical properties of the SD and Lewis urethra and have shown that the Lewis rat may not be suitable as a chronic model of SUI via nerve transection.
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影响因子：
7.2
作者：
Nygaard, IE;Heit, M
通讯作者：
Heit, M
影响因子：
1.8
作者：
Conway, DA;Kamo, I;Cannon, TW
通讯作者：
Cannon, TW
DOI：
10.1682/jrrd.2003.11.0169
发表时间：
2005-01-01
影响因子：
--
作者：
Ahn, H;Lin, DL;Damaser, MS
通讯作者：
Damaser, MS
DOI：
10.1152/ajprenal.00330.2005
发表时间：
2006-05-01
影响因子：
4.2
作者：
Jankowski, RJ;Prantil, RL;Vorp, DA
通讯作者：
Vorp, DA
影响因子：
2.6
作者：
Kinebuchi, Yoshiaki;Aizawa, Naoki;Nishizawa, Osamu
通讯作者：
Nishizawa, Osamu