Diazotroph abundance and community composition in an acidic soil in response to aluminum-tolerant and aluminum-sensitive maize (Zea mays L.) cultivars under two nitrogen fertilizer forms
Diazotroph abundance and community composition in an acidic soil in response to aluminum-tolerant and aluminum-sensitive maize (Zea mays L.) cultivars under two nitrogen fertilizer forms
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酸性土壤中固氮菌丰度和群落组成对两种氮肥形式下耐铝和铝敏感玉米（Zea mays L.）品种的响应
DOI：
10.1007/s11104-017-3550-0
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发表时间：
2018-01
期刊：
影响因子：
4.9
通讯作者：
Shen Ren Fang
中科院分区：
文献类型：
--
作者：
Wang Chao;Zheng Man Man;Hu An Yong;Zhu Chun Quan;Shen Ren Fang
在酸性土壤中，植物对铝的耐受性和氮肥形态对植物生长有重要影响。固氮菌作为促进植物生长的根际细菌，对植物有效氮有贡献，但对酸性土壤中植物耐铝性或氮肥形态对植物生长差异的响应知之甚少。在这里，我们调查了这种responsibility.MethodsThree玉米品种与不同的耐铝水平生长在酸性土壤中，添加硝酸盐（NO3−-N）或铵（NH 4 +-N）肥料。42天后，玉米的生物量和根形态，以及散装和根际土壤的理化性质进行了测定。基于nifH基因，通过定量RT-PCR测定了土壤固氮菌丰度，并采用高通量测序法测定了土壤固氮菌群落组成。结果对于同一玉米品种，施用NO3−-N肥相对于NH 4 +-N肥增加了玉米生物量、根长、根表面积和根际pH，但对根际nifH拷贝数和群落组成无显著影响。3个玉米品种根际nifH基因拷贝数差异不显著，但耐铝和铝敏感品种根际nifH基因拷贝数差异显著。
AimsIn acidic soil, plant aluminum (Al) tolerance and nitrogen (N) fertilizer form play the important roles in influencing plant growth. Diazotrophs as plant growth promoting rhizobacteria can contribute to plant available N, but the response to plant growth differences resulting from plant Al tolerance or N fertilizer form in acidic soil is poorly understood. Here, we investigated this response.MethodsThree maize cultivars with different Al-tolerance levels were grown in an acidic soil that was added with nitrate (NO3−-N) or ammonium (NH4+-N) fertilizers. After 42 days, maize biomasses and root morphologies as well as the physicochemical properties of bulk and rhizosphere soils were determined. Based on nifH gene, soil diazotroph abundance was determined by quantitative RT-PCR and community composition was assayed using high-throughput sequencing.ResultsFor the same maize cultivar, application of NO3−-N fertilizer relative to NH4+-N fertilizer increased maize biomass, root length, root surface area, and rhizosphere pH, but not significantly affect rhizosphere nifH copy number and community composition. The nifH copy numbers did not show significant differences among rhizosphere samples of three maize cultivars, but community composition was obviously different between Al-tolerant and Al-sensitive cultivars.Conclusions
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影响因子：
4.8
作者：
Zhao, Jun;Ni, Tian;Shen, Qirong
通讯作者：
Shen, Qirong
DOI：
10.1093/database/bau001
发表时间：
2014
期刊：
Database : the journal of biological databases and curation
影响因子：
--
作者：
Gaby JC;Buckley DH
通讯作者：
Buckley DH
影响因子：
9.4
作者：
W. Jackson;R. Volk
通讯作者：
W. Jackson;R. Volk
影响因子：
4.9
作者：
VONUEXKULL, HR;MUTERT, E
通讯作者：
MUTERT, E
影响因子：
4.4
作者：
Lindsay, Elizabeth A.;Colloff, Matthew J.;Wakelin, Steven A.
通讯作者：
Wakelin, Steven A.