Pandemic Swine-Origin H1N1 Influenza Virus Replicates to Higher Levels and Induces More Fever and Acute Inflammatory Cytokines in Cynomolgus versus Rhesus Monkeys and Can Replicate in Common Marmosets.
Pandemic Swine-Origin H1N1 Influenza Virus Replicates to Higher Levels and Induces More Fever and Acute Inflammatory Cytokines in Cynomolgus versus Rhesus Monkeys and Can Replicate in Common Marmosets.
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DOI：
10.1371/journal.pone.0126132
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Bogers WM
中科院分区：
文献类型：
--
作者：
Mooij P;Koopman G;Mortier D;van Heteren M;Oostermeijer H;Fagrouch Z;de Laat R;Kobinger G;Li Y;Remarque EJ;Kondova I;Verschoor EJ;Bogers WM
与人类密切的免疫学和生理学相似性使得非人灵长类动物成为研究流感病毒发病机制以及免疫和疫苗抗感染功效的有价值的模型。尽管食蟹猴和恒河猴都经常用于流感病毒研究，但尚未对感染和疾病的易感性进行直接比较。在当前的研究中，通过使用最近描述的猪源大流行 H1N1 毒株 A/Mexico/InDRE4487/2009，对这些物种进行了头对头比较。与恒河猴相比，食蟹猴在上呼吸道和肺部的病毒复制水平明显更高，包括病毒产生的峰值水平和持续时间，以及体温的更高升高。相比之下，在恒河猴中更容易观察到临床症状，包括呼吸窘迫。唾液酸-α-2,6-Gal糖是人类甲型流感病毒的主要受体，食蟹猴气管和支气管中的表达量是恒河猴的50至73倍。研究还表明，普通狨猴（一种新大陆非人类灵长类动物）很容易感染 H1N1 流感大流行。研究结果有利于食蟹猴作为大流行性甲型H1N1流感病毒研究的模型，因为食蟹猴的病毒复制更加均匀和高水平，并且温度升高，这可能是由于主要人类流感病毒受体在气管和支气管中表达更丰富。
The close immunological and physiological resemblance with humans makes non-human primates a valuable model for studying influenza virus pathogenesis and immunity and vaccine efficacy against infection. Although both cynomolgus and rhesus macaques are frequently used in influenza virus research, a direct comparison of susceptibility to infection and disease has not yet been performed. In the current study a head-to-head comparison was made between these species, by using a recently described swine-origin pandemic H1N1 strain, A/Mexico/InDRE4487/2009. In comparison to rhesus macaques, cynomolgus macaques developed significantly higher levels of virus replication in the upper airways and in the lungs, involving both peak level and duration of virus production, as well as higher increases in body temperature. In contrast, clinical symptoms, including respiratory distress, were more easily observed in rhesus macaques. Expression of sialyl-α-2,6-Gal saccharides, the main receptor for human influenza A viruses, was 50 to 73 times more abundant in trachea and bronchus of cynomolgus macaques relative to rhesus macaques. The study also shows that common marmosets, a New World non-human primate species, are susceptible to infection with pandemic H1N1. The study results favor the cynomolgus macaque as model for pandemic H1N1 influenza virus research because of the more uniform and high levels of virus replication, as well as temperature increases, which may be due to a more abundant expression of the main human influenza virus receptor in the trachea and bronchi.
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影响因子：
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作者：
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