Genomic insights into the serine protease gene family and expression profile analysis in the planthopper, Nilaparvata lugens.
Genomic insights into the serine protease gene family and expression profile analysis in the planthopper, Nilaparvata lugens.
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飞虱丝氨酸蛋白酶基因家族的基因组学见解和表达谱分析
DOI：
10.1186/1471-2164-15-507
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发表时间：
2014-06-21
期刊：
影响因子：
4.4
通讯作者：
Zhang CX
中科院分区：
文献类型：
--
作者：
Bao YY;Qin X;Yu B;Chen LB;Wang ZC;Zhang CX
褐飞虱是亚洲最具破坏性的水稻害虫之一。褐飞虱通过吸入水稻韧皮部汁液对水稻造成广泛的危害，从而导致漏斗烧伤(水稻植株完全死亡)。尽管它很重要，但人们对这种半代谢害虫的消化、发育和防御机制知之甚少。在这项研究中，我们的目标是鉴定丝氨酸蛋白酶(SP)和丝氨酸蛋白酶同源基因(SPH)，这两个基因在真核生物中形成了一个大家族，因为它们具有多种生理功能。拥有全序列的褐飞虱基因组使我们能够深入分析基因结构，揭示进化关系，预测SP基因的生理功能。全基因组和转录组分析确定了褐飞虱中90个可能的SP(65)和SPH(25)基因。关于基因组中外显子-内含子的组织、大小、分布和转录方向的详细基因信息表明，多个SP/SPH基因座紧密位于同一支架上，表明在这个大的基因家族中经常发生基因重复。基因表达谱揭示了关于SP/SPH如何应对细菌感染以及它们的组织、发育和性别特异性表达的新发现。我们的发现提供了全面的褐飞虱SP和SPH基因的基因序列资源和表达谱，这为阐明这些基因在发育、消化、繁殖和免疫等生物过程中的潜在功能提供了见解。本文的在线版本(DOI：10.1186/1471-2164-15-507)包含补充材料，授权用户可以使用。
The brown planthopper (Nilaparvata lugens) is one of the most destructive rice plant pests in Asia. N. lugens causes extensive damage to rice by sucking rice phloem sap, which results in hopper burn (complete death of the rice plants). Despite its importance, little is known about the digestion, development and defense mechanisms of this hemimetabolous insect pest. In this study, we aim to identify the serine protease (SP) and serine protease homolog (SPH) genes, which form a large family in eukaryotes, due to the potential for multiple physiological roles. Having a fully sequenced genome for N. lugens allows us to perform in-depth analysis of the gene structures, reveal the evolutionary relationships and predict the physiological functions of SP genes. The genome- and transcriptome-wide analysis identified 90 putative SP (65) and SPH (25) genes in N. lugens. Detailed gene information regarding the exon-intron organization, size, distribution and transcription orientation in the genome revealed that many SP/SPH loci are closely situated on the same scaffold, indicating the frequent occurrence of gene duplications in this large gene family. The gene expression profiles revealed new findings with regard to how SPs/SPHs respond to bacterial infections as well as their tissue-, development- and sex-specific expressions. Our findings provide comprehensive gene sequence resources and expression profiles of the N. lugens SP and SPH genes, which give insights into clarifying the potentially functional roles of these genes in the biological processes including development, digestion, reproduction and immunity. The online version of this article (doi:10.1186/1471-2164-15-507) contains supplementary material, which is available to authorized users.
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褐飞虱 Nilaparvata lugens 先天免疫的全基因组和转录组分析
影响因子：
4.4
作者：
Bao YY;Qu LY;Zhao D;Chen LB;Jin HY;Xu LM;Cheng JA;Zhang CX
通讯作者：
Zhang CX
影响因子：
2.6
作者：
Bao, Y-Y.;Lv, Z-Y.;Zhang, C-X.
通讯作者：
Zhang, C-X.
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2.6
作者：
Zou Z;Lopez DL;Kanost MR;Evans JD;Jiang H
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Jiang H
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3.8
作者：
Yu, XQ;Jiang, HB;Kanost, MR
通讯作者：
Kanost, MR
影响因子：
4.8
作者：
Kan, Hongnan;Kim, Chan-Hee;Lee, Bok Luel
通讯作者：
Lee, Bok Luel