The genome- and transcriptome-wide analysis of innate immunity in the brown planthopper, Nilaparvata lugens.
The genome- and transcriptome-wide analysis of innate immunity in the brown planthopper, Nilaparvata lugens.
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褐飞虱 Nilaparvata lugens 先天免疫的全基因组和转录组分析
DOI：
10.1186/1471-2164-14-160
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发表时间：
2013-03-09
期刊：
影响因子：
4.4
通讯作者：
Zhang CX
中科院分区：
文献类型：
--
作者：
Bao YY;Qu LY;Zhao D;Chen LB;Jin HY;Xu LM;Cheng JA;Zhang CX
背景
褐飞虱（Nilaparvata lugens）是亚洲最严重的水稻害虫之一。N.褐飞虱通过吸食水稻韧皮部汁液而引起水稻的广泛损害，这导致植物生长受阻和植物病毒的传播。尽管这种昆虫害虫的重要性，很少有人知道发生在这种半代谢昆虫物种的免疫机制。
结果
在这项研究中，我们进行了基因组和转录组范围内的分析，针对免疫相关基因。转录组数据集包括N.褐飞虱肠、发育阶段、翅形成和性别特异性表达信息，为全基因组分析提供了有用的基因表达序列数据。结果，我们鉴定了大量编码N.褐变模式识别蛋白、酚氧化酶原（proPO）激活级联中的调节蛋白、免疫效应物和参与免疫途径的信号转导分子，包括Toll、免疫缺陷（Imd）和Janus激酶信号转导子和转录激活子（JAK-STAT）途径。基因组规模分析揭示了支架中基因结构、分布和转录方向的详细信息。半代谢昆虫和代谢昆虫的基因组比较表明了免疫相关基因构成的差异。我们研究了基因表达谱，以了解它们如何对细菌感染和组织做出反应，以及发育和性别表达特异性。
结论
免疫相关基因的全基因组和转录组分析，包括模式识别和调节分子，免疫效应物，以及参与免疫途径的信号转导分子，是确定N. lugens。本研究结果提供了完整的N.褐飞虱，这有助于了解半代谢昆虫物种的先天免疫机制。这些数据有助于阐明免疫相关基因在发育、繁殖和病毒传播等生物学过程中的潜在功能。lugens。
Background
The brown planthopper (Nilaparvata lugens) is one of the most serious rice plant pests in Asia. N. lugens causes extensive rice damage by sucking rice phloem sap, which results in stunted plant growth and the transmission of plant viruses. Despite the importance of this insect pest, little is known about the immunological mechanisms occurring in this hemimetabolous insect species.
Results
In this study, we performed a genome- and transcriptome-wide analysis aiming at the immune-related genes. The transcriptome datasets include the N. lugens intestine, the developmental stage, wing formation, and sex-specific expression information that provided useful gene expression sequence data for the genome-wide analysis. As a result, we identified a large number of genes encoding N. lugens pattern recognition proteins, modulation proteins in the prophenoloxidase (proPO) activating cascade, immune effectors, and the signal transduction molecules involved in the immune pathways, including the Toll, Immune deficiency (Imd) and Janus kinase signal transducers and activators of transcription (JAK-STAT) pathways. The genome scale analysis revealed detailed information of the gene structure, distribution and transcription orientations in scaffolds. A comparison of the genome-available hemimetabolous and metabolous insect species indicate the differences in the immune-related gene constitution. We investigated the gene expression profiles with regards to how they responded to bacterial infections and tissue, as well as development and sex expression specificity.
Conclusions
The genome- and transcriptome-wide analysis of immune-related genes including pattern recognition and modulation molecules, immune effectors, and the signal transduction molecules involved in the immune pathways is an important step in determining the overall architecture and functional network of the immune components in N. lugens. Our findings provide the comprehensive gene sequence resource and expression profiles of the immune-related genes of N. lugens, which could facilitate the understanding of the innate immune mechanisms in the hemimetabolous insect species. These data give insight into clarifying the potential functional roles of the immune-related genes involved in the biological processes of development, reproduction, and virus transmission in N. lugens.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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DOI：
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10.1016/s0006-291x(03)01096-9
发表时间：
2003-07-11
影响因子：
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作者：
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