Differing structures of galactoglucomannan in eudicots and non-eudicot angiosperms
Differing structures of galactoglucomannan in eudicots and non-eudicot angiosperms
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真双子叶植物和非真双子叶被子植物中半乳葡甘露聚糖的不同结构
DOI:
10.1101/2023.06.18.545452
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发表时间:
2023
期刊:
影响因子:
--
通讯作者:
Ishida K
中科院分区:
文献类型:
--
作者:
Ishida K
The structures of cell wall mannan hemicelluloses have changed during plant evolution. Recently, a new structure called β-galactoglucomannan (β-GGM) was discovered in eudicot plants. This galactoglucomannan has β-(1,2)-Gal-α-(1,6)-Gal disaccharide branches on some mannosyl residues of the strictly alternating Glc-Man backbone. Studies in Arabidopsis revealed β-GGM is related in structure, biosynthesis and function to xyloglucan. However, when and how plants acquired β-GGM remains elusive. Here, we studied mannan structures in many sister groups of eudicots. All glucomannan structures were distinct from β-GGM. In addition, we searched for candidate mannan β-galactosyltransferases (MBGT) in non-eudicot angiosperms. CandidateAtMBGT1 orthologues from rice (OsGT47A-VII) and Amborella (AtrGT47A-VII) did not show MBGT activityin vivo. However, theAtMBGT1 orthologue from rice showed MUR3-like xyloglucan galactosyltransferase activity in complementation analysis using Arabidopsis. Further, reverse genetic analysis revealed that the enzyme (OsGT47A-VII) contributes to proper root growth in rice. Together, gene duplication and diversification of GT47A-VII in eudicot evolution may have been involved in the acquisition of mannan β-galactosyltransferase activity. Our results indicate that β-GGM is likely to be a eudicot-specific mannan.
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影响因子:
11.2
作者:
Yves Verhertbruggen;X. Falourd;Marlayna Sterner;F. Guillon;C. Girousse;L. Foucat;S. Le Gall;A. Chateigner-Boutin;L. Saulnier
通讯作者:
Yves Verhertbruggen;X. Falourd;Marlayna Sterner;F. Guillon;C. Girousse;L. Foucat;S. Le Gall;A. Chateigner-Boutin;L. Saulnier
DOI:
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2009
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影响因子:
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通讯作者:
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