Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster.
Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0025025
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Šimek P
中科院分区：
文献类型：
--
作者：
Koštál V;Korbelová J;Rozsypal J;Zahradníčková H;Cimlová J;Tomčala A;Šimek P
黑腹果蝇是一种容易受寒的昆虫。以前对这种蝇的研究主要集中在冷休克期间的急性直接冷伤，并表明较低的致死温度(−5°C)表现出相对较低的可塑性，而且无论是快速冷锻炼还是长期冷驯化，较低的致死温度最大仅使致死温度移动几度。我们发现，长期的冷驯化显著提高了完全发育的黑腹水母三龄幼虫的耐冷性。25℃恒温驯化与15℃恒温驯化2 d(15℃→6℃)的比较表明，长期冷驯化使50%的幼虫在0℃恒温条件下的致死时间延长了630倍(从0.137 h延长到86.658 h)。这种显著的生理可塑性(与LLT相反)表明，0℃下的慢性间接冷害不同于冷休克引起的冷害。长期冷驯化改变了幼虫的代谢谱。最显著的两种反应是脯氨酸(高达17.7 mm)和海藻糖(高达36.5 mm)。此外，观察到生物膜甘油磷脂组成的重组。甘油磷乙醇胺(特别是那些在sn-2位含有亚油酸的甘油磷乙醇胺)的相对比例增加，而甘油磷胆碱的比例下降。黑腹小食心虫3龄幼虫对长期冷驯化的反应提高了其耐寒性，并显示出积累脯氨酸和海藻糖以及膜重组的代谢潜力。
Drosophila melanogaster is a chill-susceptible insect. Previous studies on this fly focused on acute direct chilling injury during cold shock and showed that lower lethal temperature (LLT, approximately −5°C) exhibits relatively low plasticity and that acclimations, both rapid cold hardening (RCH) and long-term cold acclimation, shift the LLT by only a few degrees at the maximum. We found that long-term cold acclimation considerably improved cold tolerance in fully grown third-instar larvae of D. melanogaster. A comparison of the larvae acclimated at constant 25°C with those acclimated at constant 15°C followed by constant 6°C for 2 d (15°C→6°C) showed that long-term cold acclimation extended the lethal time for 50% of the population (Lt50) during exposure to constant 0°C as much as 630-fold (from 0.137 h to 86.658 h). Such marked physiological plasticity in Lt50 (in contrast to LLT) suggested that chronic indirect chilling injury at 0°C differs from that caused by cold shock. Long-term cold acclimation modified the metabolomic profiles of the larvae. Accumulations of proline (up to 17.7 mM) and trehalose (up to 36.5 mM) were the two most prominent responses. In addition, restructuring of the glycerophospholipid composition of biological membranes was observed. The relative proportion of glycerophosphoethanolamines (especially those with linoleic acid at the sn-2 position) increased at the expense of glycerophosphocholines. Third-instar larvae of D. melanogaster improved their cold tolerance in response to long-term cold acclimation and showed metabolic potential for the accumulation of proline and trehalose and for membrane restructuring.
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影响因子：
2.9
作者：
HOFFMANN, AA;WATSON, M
通讯作者：
WATSON, M
影响因子：
2.2
作者：
DUMAN, JG
通讯作者：
DUMAN, JG
影响因子：
4.1
作者：
Hincha, DK;Hagemann, M
通讯作者：
Hagemann, M
影响因子：
2.2
作者：
CHEN, CP;WALKER, VK
通讯作者：
WALKER, VK
影响因子：
2.2
作者：
Hodkova, M;Berková, P;Zahradnícková, H
通讯作者：
Zahradnícková, H