Profiling membrane glycerolipids during γ-ray-induced membrane injury.
Profiling membrane glycerolipids during γ-ray-induced membrane injury.
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伽马射线诱导的膜损伤过程中膜甘油脂的分析
DOI：
10.1186/s12870-017-1153-9
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发表时间：
2017-11-15
影响因子：
5.3
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Zheng G;Li W
γ 射线是高能辐射，会对植物细胞造成一系列随机损伤。关于这个问题的大多数研究都集中在γ射线诱导的核苷酸损伤和细胞内活性氧的产生上，因此对γ射线诱导的膜损伤过程中甘油脂代谢知之甚少。使用基于 ESI-MS/MS 的脂质组学方法，我们分析了野生型和磷脂酶 D (PLD)δ 和 α1 缺陷拟南芥在 γ 射线处理后的脂质组变化。本研究的目的是探讨 PLD 介导的甘油脂代谢在 γ 射线诱导的膜损伤中的作用。拟南芥叶片经2885Gyγ射线处理后离子泄漏小于10%。高剂量γ射线治疗可诱导细胞内活性氧（ROS）的积累。 PLDα1 的抑制导致 γ 射线治疗下严重的脂质降解。 γ射线诱导的甘油脂降解主要发生在叶绿体脂质中，而不是质体外脂质。在 γ 射线处理期间，WS 生态型中的溶血磷脂酰胆碱 (lysoPC) 和溶血磷脂酰乙醇胺 (lysoPE) 水平保持不变，而在经 1100 Gy 处理的 Col 生态型中则显着增加。经过210Gy和1100Gyγ射线处理后，四种基因型拟南芥的溶血磷脂酰甘油（lysoPG）水平显着下降。 γ射线诱导的膜损伤可能通过间接机制发生。不同脂质的降解不是同步的，并且脂质之间可能发生相互转化。在 γ 射线诱导的膜损伤过程中，磷脂酰胆碱 (PC) 和磷脂酰乙醇胺 (PE) 的降解可能是由 PLDζ1 或磷脂酶 A1 介导的。磷脂酰甘油的降解不是由PLA、PLDδ或PLDα1介导的，而是由磷脂酶C或其他PLD介导的。 γ射线可以降低膜脂中的双键指数并增加酰基链长度，这可能使膜变得更加坚硬，并进一步引起膜损伤。本文的在线版本 (10.1186/s12870-017-1153-9) 包含补充材料，可供授权用户使用。
γ-rays are high-energy radiation that cause a range of random injuries to plant cells. Most studies on this issue have focused on γ-ray-induced nucleotide damage and the production of reactive oxygen species in cells, so little is known about the glycerolipid metabolism during γ-rays induced membrane injury. Using an ESI-MS/MS-based lipidomic method, we analysed the lipidome changes in wild-type and phospholipase D (PLD)δ- and α1-deficient Arabidopsis after γ-ray treatment. The aim of this study was to investigate the role of PLD-mediated glycerolipid metabolism in γ-ray-induced membrane injury. The ion leakage of Arabidopsis leaves after 2885-Gy γ-ray treatment was less than 10%. High does γ-ray treatment could induce the accumulation of intracellular reactive oxygen species (ROS). Inhibition of PLDα1 caused severe lipid degradation under γ-ray treatment. γ-ray-induced glycerolipid degradation mostly happened in chloroplastidic lipids, rather than extraplastidic ones. The levels of lysophosphatidylcholine (lysoPC) and lysophosphatidylethanolamine (lysoPE) were maintained in the WS ecotypes during γ-ray treatments, while increased significantly in the Col ecotype treated with 1100 Gy. After 210- and 1100-Gy γ-ray treatments, the level of lysophosphatidylglycerol (lysoPG) decreased significantly in the four genotypes of Arabidopsis. γ-ray-induced membrane injury may occur via an indirect mechanism. The degradation of distinct lipids is not synchronous, and that interconversions among lipids can occur. During γ-ray-induced membrane injury, the degradation of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) may be mediated by PLDζ1 or phospholipase A1. The degradation of phosphatidylglycerol was not mediated by PLA, PLDδ or PLDα1, but by phospholipase C or other PLDs. γ-rays can decrease the double-bond index and increase the acyl chain length in membrane lipids, which may make membranes more rigid and further cause injury in membranes. The online version of this article (10.1186/s12870-017-1153-9) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Jia Y;Tao F;Li W
通讯作者：
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拟南芥冷冻和冷冻后恢复过程中质体外和质体脂质的差异降解
影响因子：
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作者：
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