Chromatin remodelers fine-tune H3K36me-directed deacetylation of neighbor nucleosomes by Rpd3S.
Chromatin remodelers fine-tune H3K36me-directed deacetylation of neighbor nucleosomes by Rpd3S.
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DOI：
10.1016/j.molcel.2013.08.024
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发表时间：
2013-10-24
期刊：
影响因子：
16
通讯作者：
Li, Bing
中科院分区：
文献类型：
--
作者：
Lee, Chul-Hwan;Wu, Jun;Li, Bing
染色质重塑剂与组蛋白修饰复合物的调节有关。然而，对潜在的机制仍然知之甚少。Rpd3S组蛋白去乙酰化酶复合物通过延长RNA聚合酶II以依赖于组蛋白3（H3K36me）上赖氨酸36的甲基化的方式去除编码区的组蛋白乙酰化而募集，并且Rpd3S偏好双核体。在这里，我们表明，结合Rpd3S的dinucleosomes和它的催化活性是敏感的核小体接头的长度在一个非线性的方式。有趣的是，我们发现当两个核小体在最佳距离内时，一个核小体上的H3K36me刺激Rpd3S使相邻的核小体脱乙酰化。最后，我们证明，染色质重塑增强Rpd3S活性通过改变核小体间距，这表明染色质重塑总理染色质配置微调随后的组蛋白修饰反应。这一机制对于在转录延伸周期中准确控制染色质动态是重要的。
Chromatin remodelers have been implicated in the regulation of histone modifying complexes. However the underlying mechanism remains poorly understood. The Rpd3S histone deacetylase complex is recruited by elongating RNA polymerase II to remove histone acetylation at coding regions in a manner that is dependent on methylation of lysine 36 on histone 3 (H3K36me), and Rpd3S prefers dinucleosomes. Here, we show that the binding of Rpd3S to dinucleosomes and its catalytic activity are sensitive to the length of nucleosomal linker in a nonlinear fashion. Intriguingly, we found that H3K36me on one nucleosome stimulates Rpd3S to deacetylate the neighboring nucleosomes when those two nucleosomes are within an optimal distance. Finally, we demonstrate that chromatin remodelers enhance Rpd3S activity by altering nucleosomal spacing, suggesting that chromatin remodelers prime chromatin configuration to fine-tune subsequent histone modification reactions. This mechanism is important for accurate temporal control of chromatin dynamics during the transcription elongation cycle.
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影响因子：
7.7
作者：
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通讯作者：
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发表时间：
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期刊：
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