Energy restriction only slightly influences protein metabolism in obese rats, whatever the level of protein and its source in the diet.
Energy restriction only slightly influences protein metabolism in obese rats, whatever the level of protein and its source in the diet.
复制标题
无论饮食中的蛋白质水平及其来源如何，能量限制仅轻微影响肥胖大鼠的蛋白质代谢。
DOI：
--
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发表时间：
2013
影响因子：
4.9
通讯作者：
C. Gaudichon
中科院分区：
文献类型：
--
作者：
L. Chevalier;C. Bos;D. Azzout;G. Fromentin;L. Mosoni;Nouredine Hafnaoui;J. Piedcoq;D. Tomé;C. Gaudichon
背景
BACKGROUND
High protein (HP) diets during energy restriction have been studied extensively regarding their ability to reduce body fat and preserve lean body mass, but little is known about their effects on protein metabolism in lean tissues.
OBJECTIVE
To determine the effects of energy restriction and protein intake on protein anabolism and catabolism in rats.
METHODS
For 5 weeks, 56 male Wistar rats were fed an obesity induction (OI) diet . They were then subjected to a 40% energy restriction using the OI diet or a balanced HP diet for 3 weeks, whereas a control group was fed the OI diet ad libitum (n=8 per group). HP-restricted rats were divided into five groups differing only in terms of their protein source: total milk proteins, casein (C), whey (W), a mix of 50% C and W, and soy (n=8). The animals were then killed in the postprandial state and their body composition was determined. Protein synthesis rates were determined in the liver, gastrocnemius and kidney using a subcutaneous (13)C valine flooding dose. mRNA levels were measured for key enzymes involved in the three proteolysis pathways.
RESULTS
Energy restriction, but not diet composition, impacted weight loss and adiposity, whereas lean tissue mass (except in the kidney) was not influenced by diet composition. Levels of neoglucogenic amino acids tended to fall under energy restriction (P<0.06) but this was reversed by a high level of protein. The postprandial protein synthesis rates in different organs were similar in all groups. By contrast, mRNA levels encoding proteolytic enzymes rose under energy restriction in the muscle and kidney, but this was counteracted by a HP level.
CONCLUSIONS
In adult obese rats, energy restriction but not diet composition affected fat pads and had little impact on protein metabolism, despite marked effects on proteolysis in the kidney and muscle.
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长期有氧运动和能量限制对蛋白质合成的影响。
DOI：
10.1016/0026-0495(95)90263-5
发表时间：
1995
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Katzeff,HL;Ojamaa,KM;Klein,I
通讯作者：
Klein,I
DOI：
10.1016/0026-0495(91)90228-o
发表时间：
1991-05
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
T. Stein;W. Rumpler;M. Leskiw;M. Schluter;R. Staples;C. E. Bodwell
通讯作者：
T. Stein;W. Rumpler;M. Leskiw;M. Schluter;R. Staples;C. E. Bodwell
DOI：
10.1152/ajpregu.00463.2004
发表时间：
2004-12-01
影响因子：
2.8
作者：
MacLean, PS;Higgins, JA;Hill, JO
通讯作者：
Hill, JO
人类丙氨酸代谢对膳食蛋白质和能量摄入控制的反应。
DOI：
10.1152/ajpendo.1986.250.1.e39
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Yang,RD;Matthews,DE;Bier,DM;Wen,ZM;Young,VR
通讯作者：
Young,VR