Genome-wide identification, phylogeny, duplication, and expression analyses of two-component system genes in Chinese cabbage (Brassica rapa ssp. pekinensis).
Genome-wide identification, phylogeny, duplication, and expression analyses of two-component system genes in Chinese cabbage (Brassica rapa ssp. pekinensis).
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DOI：
10.1093/dnares/dsu004
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Liu Z;Zhang M;Kong L;Lv Y;Zou M;Lu G;Cao J;Yu X
在植物中，由组氨酸传感激酶（HKs）、组氨酸磷酸转移蛋白（HPs）和反应调节因子（RRs）组成的双组分系统（TCS）已被用于细胞分裂素信号转导。TCS在多种生物过程中表现出重要功能，包括植物生长、发育和对环境刺激的响应。对大白菜基因组进行了详尽的搜索，共鉴定出20个HK（L）（11个HK和9个HKL）、8个HP（7个真实的和1个假的）和57个RR（21个Type-A、17个Type-B、4个Type-C和15个假的）蛋白。详细分析了这些蛋白质编码基因的结构、保守结构域和系统发育关系。TCS基因的重复，进化模式和分歧进行了调查。进一步分析TCS基因在不同组织、器官和发育阶段中的转录水平，以获得这些基因功能的信息。细胞分裂素相关的结合元件被发现在推定的启动子区域的A型BrRR基因。此外，基因表达模式的不利环境胁迫（干旱和高盐）和外源植物激素（tZ和阿坝）进行了研究。获得了大量的胁迫响应候选基因。本研究的系统分析为进一步了解大白菜TCS基因的特性和功能奠定了基础。
In plants, a two component system (TCS) composed of sensor histidine kinases (HKs), histidine phosphotransfer proteins (HPs), and response regulators (RRs) has been employed in cytokinin signal transduction. A TCS exhibits important functions in diverse biological processes, including plant growth, development, and response to environmental stimuli. Conducting an exhaustive search of the Chinese cabbage genome, a total of 20 HK(L) (11 HKs and 9 HKLs), 8 HP (7 authentic and 1 pseudo), and 57 RR (21 Type-A, 17 Type-B, 4 Type-C, and 15 pseudo) proteins were identified. The structures, conserved domains, and phylogenetic relationships of these protein-coding genes were analysed in detail. The duplications, evolutionary patterns, and divergence of the TCS genes were investigated. The transcription levels of TCS genes in various tissues, organs, and developmental stages were further analysed to obtain information of the functions of these genes. Cytokinin-related binding elements were found in the putative promoter regions of Type-A BrRR genes. Furthermore, gene expression patterns to adverse environmental stresses (drought and high salinity) and exogenous phytohormones (tZ and ABA) were investigated. Numerous stress-responsive candidate genes were obtained. Our systematic analyses provided insights into the characterization of the TCS genes in Chinese cabbage and basis for further functional studies of such genes.
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影响因子：
7
作者：
Blanc, G;Hokamp, K;Wolfe, KH
通讯作者：
Wolfe, KH
DOI：
10.1093/dnares/dst012
发表时间：
2013-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Behnam B;Iuchi S;Fujita M;Fujita Y;Takasaki H;Osakabe Y;Yamaguchi-Shinozaki K;Kobayashi M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
6.9
作者：
Caesar K;Thamm AM;Witthöft J;Elgass K;Huppenberger P;Grefen C;Horak J;Harter K
通讯作者：
Harter K
影响因子：
5.1
作者：
Fusada, N;Masuda, T;Takamiya, K
通讯作者：
Takamiya, K
DOI：
10.1073/pnas.0909766107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Beilstein, Mark A.;Nagalingum, Nathalie S.;Mathews, Sarah
通讯作者：
Mathews, Sarah