Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry.
Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry.
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DOI：
10.1038/s41467-018-04284-8
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发表时间：
2018-05-16
影响因子：
16.6
通讯作者：
Fürthauer M
中科院分区：
文献类型：
--
作者：
Juan T;Géminard C;Coutelis JB;Cerezo D;Polès S;Noselli S;Fürthauer M
左右（LR）不对称的建立是动物发育的基础，但在不同门之间建立侧向性的统一机制仍然难以捉摸。纤毛驱动的定向流体流动对包括斑马鱼在内的许多脊椎动物的对称性破坏很重要。或者，LR不对称可以独立于纤毛建立，特别是通过肌动蛋白细胞骨架的固有手性。在这里，我们发现肌球蛋白1d （Myo1D）是一种先前确定的果蝇LR不对称调节因子，对斑马鱼LR组织者（LRO）库普弗囊泡（KV）的形成和功能至关重要。Myo1D控制LRO纤毛的方向，并与平面细胞极性（PCP）通路组分VanGogh-like2 （Vangl2）相互作用，形成高效的LRO流。我们的研究结果确定Myo1D是动物LR不对称的进化保守调节剂，并表明Myo1D和PCP之间的功能相互作用是动物LR不对称建立的核心。左右（LR）轴的规格是必不可少的胚胎模式，但一个统一的机制，跨生物体尚未确定。在这里，作者表明Myosin1D，已知调节果蝇LR不对称，控制斑马鱼LR组织者功能，因此是动物侧性的保守调节剂。
The establishment of left–right (LR) asymmetry is fundamental to animal development, but the identification of a unifying mechanism establishing laterality across different phyla has remained elusive. A cilia-driven, directional fluid flow is important for symmetry breaking in numerous vertebrates, including zebrafish. Alternatively, LR asymmetry can be established independently of cilia, notably through the intrinsic chirality of the acto-myosin cytoskeleton. Here, we show that Myosin1D (Myo1D), a previously identified regulator of Drosophila LR asymmetry, is essential for the formation and function of the zebrafish LR organizer (LRO), Kupffer’s vesicle (KV). Myo1D controls the orientation of LRO cilia and interacts functionally with the planar cell polarity (PCP) pathway component VanGogh-like2 (Vangl2), to shape a productive LRO flow. Our findings identify Myo1D as an evolutionarily conserved regulator of animal LR asymmetry, and show that functional interactions between Myo1D and PCP are central to the establishment of animal LR asymmetry. Left-right (LR) axis specification is essential for embryonic patterning but a unifying mechanism across organisms has not been identified. Here, the authors show that Myosin1D, known to regulate Drosophila LR asymmetry, controls zebrafish LR Organizer function, and is therefore a conserved regulator of animal laterality.
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影响因子：
11.8
作者：
Compagnon, Julien;Barone, Vanessa;Heisenberg, Carl-Philipp
通讯作者：
Heisenberg, Carl-Philipp
DOI：
10.1073/pnas.1308335110
发表时间：
2013-08-20
影响因子：
11.1
作者：
Jao, Li-En;Wente, Susan R.;Chen, Wenbiao
通讯作者：
Chen, Wenbiao
影响因子：
64.5
作者：
Levin, M;Thorlin, T;Mercola, M
通讯作者：
Mercola, M
影响因子：
3.7
作者：
Antic D;Stubbs JL;Suyama K;Kintner C;Scott MP;Axelrod JD
通讯作者：
Axelrod JD
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF