Frog Skin Derived Peptides With Potential Protective Effects on Ultraviolet B-Induced Cutaneous Photodamage.
Frog Skin Derived Peptides With Potential Protective Effects on Ultraviolet B-Induced Cutaneous Photodamage.
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DOI：
10.3389/fimmu.2021.613365
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发表时间：
2021
影响因子：
7.3
通讯作者：
Liang S
中科院分区：
文献类型：
--
作者：
Liu H;Guo X;Yi T;Zhu Y;Ren X;Guo R;Dai Y;Liang S
红腹雨蛙是一种生活在我国西南高原地区的树蛙，该地区紫外线强，日照时间长。因此，它们裸露的皮肤可能具有化学防御成分，可以保护它免受急性光损伤。然而，迄今为止还没有鉴定出这样的肽或组分。本研究从树蛙皮肤中分离到两种新的多肽（FW-1，FWPMI-NH 2和FW-2，FWPMI-NH 2）。FW-1的5个拷贝和FW-2的4个拷贝由相同的基因编码，并从相同的蛋白质前体释放，其具有167个氨基酸残基。FW-1和FW-2通过直接抑制UVB诱导的炎症细胞因子如肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的分泌而发挥显著的抗炎作用。它们可能通过调节UV诱导的应激信号通路如丝裂原活化蛋白激酶（MAPK）和核因子κ B（NF-κB）来实现这一功能。此外，FW-1和FW-2通过抑制UVB诱导的活性氧（ROS）的产生，对表皮具有潜在的抗氧化作用，其机制尚不清楚。考虑到小肽易于生产、储存和潜在的光保护活性，FW-1/2可能是开发用于抑制UVB诱导的皮肤炎症的新型药理学试剂的令人兴奋的先导化合物或模板。此外，本研究还为进一步研究紫外线对树蛙皮肤的防御机制提供了理论依据。
Hyla annectans is a tree frog living in the southwestern plateau area of China where there is strong ultraviolet radiation and long duration of sunshine. So their naked skin may possess chemical defense components that protect it from acute photo-damage. However, no such peptide or components has been identified till to date. In the current work, two novel peptides (FW-1, FWPLI-NH2 and FW-2, FWPMI-NH2) were identified from the skin of the tree frog. Five copies of FW-1 and four copies of FW-2 are encoded by an identical gene and released from the same protein precursor, which possess 167 amino acid residues. FW-1 and -2 can exert significant anti-inflammatory functions by directly inhibiting Ultraviolet B irradiation (UVB)-induced secretion of inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). They may achieve this function by modulating the UV-induced stress signaling pathways such as Mitogen-activated protein kinases (MAPK) and Nuclear Factor Kappa B (NF-κB). Besides, FW-1 and -2 showed potential antioxidant effects on epidermis by attenuating the UVB-induced reactive oxygen species (ROS) production through an unknown mechanism. Considering small peptides’ easy production, storage, and potential photo-protective activity, FW-1/2 might be exciting leading compounds or templates for the development of novel pharmacological agents for the suppression of UVB-induced skin inflammation. Moreover, this study might expand our knowledge on skin defensive mechanism of tree frog upon UVB irradiation.
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DOI：
10.1083/jcb.133.1.211
发表时间：
1996-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang RP;Wu JX;Fan Y;Adamson ED
通讯作者：
Adamson ED
DOI：
10.1006/bbrc.1995.1067
发表时间：
1995-01-17
影响因子：
3.1
作者：
MASAKI, H;ATSUMI, T;SAKURAI, H
通讯作者：
SAKURAI, H
影响因子：
6.5
作者：
Assefa, Z;Garmyn, M;Agostinis, P
通讯作者：
Agostinis, P
影响因子：
3.3
作者：
Janda, Jaroslav;Burkett, Nichole B.;Dickinson, Sally E.
通讯作者：
Dickinson, Sally E.
影响因子：
10.3
作者：
COX, NH;DIFFEY, BL;FARR, PM
通讯作者：
FARR, PM