3-D printing provides a novel approach for standardization and reproducibility of freezing devices.
3-D printing provides a novel approach for standardization and reproducibility of freezing devices.
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DOI：
10.1016/j.cryobiol.2017.03.010
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发表时间：
2017-06
期刊：
影响因子：
2.7
通讯作者：
Tiersch TR
中科院分区：
文献类型：
--
作者：
Hu E;Childress W;Tiersch TR
超低温保存已成为动植物种质资源长期保存的重要手段。为了保护遗传资源，在国家和国际合作下建立了储存库。为了使存放处发挥作用，提交的遗传材料必须质量良好，并与其他提交的材料具有可比性。然而，由于各种原因，包括知识和可用资源的限制，水生物种的冷冻保存方法在用户群体中差异很大，这降低了重现性并削弱了质量控制。在这里，我们描述了一个可扩展的冷冻装置，使用三维（3-D）打印，并引入网络共享的概念，以实现聚集体高通量冷冻保存水生物种。其目标是：1）采用广泛可用的聚苯乙烯泡沫产品，其将是廉价的、便携的，并且提供足够的工作空间; 2）开发适合于3D打印的设计，其可以提供多种配置，是廉价的，并且易于使用，以及3）评估各种配置以获得适合于各种常见的低温保存容器的冷冻速率。通过这种方法，可以在全球范围内访问相同的组件，并且我们证明了3D打印机可以用于制造可拆卸冷冻设备的部件，从而在用户之间产生相关和可重现的冷却速率。有了标准化的冷冻设备，方法和样品可以协调成一个聚合的高通量途径，目前还没有提供水生物种储存库的发展。
Cryopreservation has become an important and accepted tool for long-term germplasm conservation of animals and plants. To protect genetic resources, repositories have been developed with national and international cooperation. For a repository to be effective, the genetic material submitted must be of good quality and comparable to other submissions. However, due to a variety of reasons, including constraints in knowledge and available resources, cryopreservation methods for aquatic species vary widely across user groups which reduces reproducibility and weakens quality control. Herein we describe a standardizable freezing device produced using 3-dimensional (3-D) printing and introduce the concept of network sharing to achieve aggregate high-throughput cryopreservation for aquatic species. The objectives were to: 1) adapt widely available polystyrene foam products that would be inexpensive, portable, and provide adequate work space; 2) develop a design suitable for 3-D printing that could provide multiple configurations, be inexpensive, and easy to use, and 3) evaluate various configurations to attain freezing rates suitable for various common cryopreservation containers. Through this approach, identical components can be accessed globally, and we demonstrated that 3-D printers can be used to fabricate parts for standardizable freezing devices yielding relevant and reproducible cooling rates across users. With standardized devices for freezing, methods and samples can harmonize into an aggregated high-throughput pathway not currently available for aquatic species repository development.
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