Bacterial community assembly and turnover within the intestines of developing zebrafish.
Bacterial community assembly and turnover within the intestines of developing zebrafish.
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DOI：
10.1371/journal.pone.0030603
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Yan Q;van der Gast CJ;Yu Y
大多数动物相关微生物存在于消化道群落中。这些肠道群落是由肠道栖息地的选择性压力以及宿主的基因型产生的，被视为额外的“器官”调节功能，但尚未完全在宿主身上进化。它们在提供营养、调节上皮发育和影响脊椎动物宿主的免疫力方面发挥着重要的功能。由于脊椎动物生来就没有微生物，人们对肠道细菌群落如何随着宿主的发育而组装和发育知之甚少。在生态框架内，我们研究了发育中的斑马鱼（从幼虫到成年动物）肠道栖息地内的细菌群落组装和更新。空间和时间的物种丰富度关系以及曼特尔和部分曼特尔测试表明，周转率较低，并且丰富度和组成最好通过时间而不是肠道体积（栖息地大小）或食物饮食的变化来预测。我们还观察到，斑马鱼肠道内的细菌群落是确定性组装的（通过观察到的低周转率反映出来），在斑马鱼发育的后期阶段转变为随机组装。这项研究非常重要，因为它提供了关于肠道细菌群落如何与宿主发育（从早期到成年阶段）协同组装的新见解。我们希望，通过在未来使用此类或一些更精细的方法研究这种脊椎动物模型的肠道微生物群，可以很好地为临床益处提供生态学见解。此外，这项研究还丰富了我们关于物种丰富度关系的时空关系如何形成的刚刚起步的知识，并提供了更多证据表明细菌群落的组装和动态是由确定性和随机性因素决定的。
The majority of animal associated microorganisms are present in digestive tract communities. These intestinal communities arise from selective pressures of the gut habitats as well as host's genotype are regarded as an extra ‘organ’ regulate functions that have not evolved wholly on the host. They are functionally essential in providing nourishment, regulating epithelial development, and influencing immunity in the vertebrate host. As vertebrates are born free of microorganisms, what is poorly understood is how intestinal bacterial communities assemble and develop in conjunction with the development of the host. Set within an ecological framework, we investigated the bacterial community assembly and turnover within the intestinal habitats of developing zebrafish (from larvae to adult animals). Spatial and temporal species-richness relationships and Mantel and partial Mantel tests revealed that turnover was low and that richness and composition was best predicted by time and not intestinal volume (habitat size) or changes in food diet. We also observed that bacterial communities within the zebrafish intestines were deterministically assembled (reflected by the observed low turnover) switching to stochastic assembly in the later stages of zebrafish development. This study is of importance as it provides a novel insight into how intestinal bacterial communities assemble in tandem with the host's development (from early to adult stages). It is our hope that by studying intestinal microbiota of this vertebrate model with such or some more refined approaches in the future could well provide ecological insights for clinical benefit. In addition, this study also adds to our still fledgling knowledge of how spatial and temporal species-richness relationships are shaped and provides further mounting evidence that bacterial community assembly and dynamics are shaped by both deterministic and stochastic considerations.
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