Leukocyte numbers and function in subjects eating n-3 enriched foods: selective depression of natural killer cell levels.
Leukocyte numbers and function in subjects eating n-3 enriched foods: selective depression of natural killer cell levels.
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DOI：
10.1186/ar2426
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发表时间：
2008
影响因子：
4.9
通讯作者：
Ferrante, Antonio
中科院分区：
文献类型：
--
作者：
Mukaro, Violet R.;Costabile, Maurizio;Murphy, Karen J.;Hii, Charles S.;Howe, Peter R.;Ferrante, Antonio
虽然omega-3长链多不饱和脂肪酸（n-3 LCPUFA）的消费已被推荐用于那些有炎症疾病风险的人，如类风湿性关节炎，但其抗炎作用的机制仍有待明确定义，特别是与n-3 LCPUFA的剂量和类型有关。本研究的目的是确定饮食补充后红细胞膜脂质中n-3 LCPUFA水平的变化是否与循环白细胞数量和功能的改变相关，有助于预防炎症。在一项双盲和安慰剂对照研究中，44名年龄在23至63岁之间的健康受试者食用标准或富含n-3 LCPUFA的典型加工食品，后者允许目标每日摄入1克n-3 LCPUFA。六个月后，通过流式细胞术评估外周血白细胞和亚群的比例和数量。还检查了白细胞的淋巴细胞增殖和细胞因子产生、中性粒细胞趋化性、趋化作用、杀菌、粘附和碘化活性。分析红细胞的脂肪酸含量。红细胞n-3 LCPUFA水平较高，绝对白细胞和淋巴细胞数量较低的主题消费n-3丰富的食物比对照组。中性粒细胞、单核细胞、T细胞（CD 3+）、T细胞亚群（CD 4+、CD 8+）和B细胞（CD 19+）数量无变化。然而，自然杀伤（NK）（CD 3-CD 16 + CD 56+）细胞数量在n-3补充的受试者低于对照组，并与红细胞中二十碳五烯酸或二十二碳六烯酸的量呈负相关。淋巴细胞增殖与IFN-γ和IL-2的产生无显著相关性，但n-3 LCPUFA膜含量越大，光毒素的产生越高。同样，中性粒细胞的趋化性，趋化作用，杀菌活性和粘附性并没有随着红细胞n-3 LCPUFA水平的变化而变化，但碘化反应随着n-3 LCPUFA含量的增加而减少。数据显示，经常长期食用富含n-3的食物导致NK细胞数量和中性粒细胞碘化活性降低，但淋巴细胞产生的光敏素增加。这些变化与接受n-3 LCPUFA补充的炎症性疾病患者中观察到的炎症反应和组织损伤减少一致。
While consumption of omega-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) has been recommended for those at risk of inflammatory disease such as rheumatoid arthritis, the mechanism of their anti-inflammatory effect remains to be clearly defined, particularly in relation to the dose and type of n-3 LCPUFA. The objective of this study was to determine whether varying the levels of n-3 LCPUFA in erythrocyte membrane lipids, following dietary supplementation, is associated with altered numbers and function of circulating leukocytes conducive to protection against inflammation. In a double-blind and placebo-controlled study, 44 healthy subjects aged 23 to 63 years consumed either standard or n-3 LCPUFA-enriched versions of typical processed foods, the latter allowing a target daily consumption of 1 gram n-3 LCPUFA. After six months, peripheral blood leukocyte and subpopulation proportions and numbers were assessed by flow cytometry. Leukocytes were also examined for lymphoproliferation and cytokine production, neutrophil chemotaxis, chemokinesis, bactericidal, adherence and iodination activity. Erythrocytes were analyzed for fatty-acid content. Erythrocyte n-3 LCPUFA levels were higher and absolute leukocyte and lymphocyte numbers were lower in subjects consuming n-3 enriched foods than in controls. There were no changes in the number of neutrophils, monocytes, T cells (CD3+), T-cell subsets (CD4+, CD8+) and B cells (CD19+). However, natural killer (NK) (CD3-CD16+CD56+) cell numbers were lower in n-3 supplemented subjects than in controls and were inversely related to the amount of eicosapentaenoic acid or docosahexaenoic acid in erythrocytes. No significant correlations were found with respect to lymphocyte lymphoproliferation and production of IFN-γ and IL-2, but lymphotoxin production was higher with greater n-3 LCPUFA membrane content. Similarly, neutrophil chemotaxis, chemokinesis, bactericidal activity and adherence did not vary with changes in erythrocyte n-3 LCPUFA levels, but the iodination reaction was reduced with higher n-3 LCPUFA content. The data show that regular long-term consumption of n-3 enriched foods leads to lower numbers of NK cells and neutrophil iodination activity but higher lymphotoxin production by lymphocytes. These changes are consistent with decreased inflammatory reaction and tissue damage seen in patients with inflammatory disorders receiving n-3 LCPUFA supplementation.
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影响因子：
7.1
作者：
Calder, Philip C.
通讯作者：
Calder, Philip C.
影响因子：
15.9
作者：
SPERLING, RI;BENINCASO, AI;ROBINSON, DR
通讯作者：
ROBINSON, DR
影响因子：
158.5
作者：
DONADIO, JV;BERGSTRALH, EJ;HOLLEY, KE
通讯作者：
HOLLEY, KE
影响因子：
4.4
作者：
Costabile, M;Hii, CST;Ferrante, A
通讯作者：
Ferrante, A
影响因子：
7.1
作者：
Kew, S;Banerjee, T;Calder, PC
通讯作者：
Calder, PC