Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model
Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model
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基于脱矿骨基质的微载体支架有利于大鼠模型体内血管化大骨再生
DOI：
10.1177/0885328218784370
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发表时间：
2018-06
影响因子：
2.9
通讯作者：
Zhi-Yong Zhang
中科院分区：
文献类型：
--
作者：
Qiannan Li;Wenjie Zhang;Guangdong Zhou;Yilin Cao;Wei Liu;Zhi-Yong Zhang
体内植入的细胞种植支架的新生血管不足仍然是工程化骨形成临床转化的主要瓶颈。脱钙骨基质具有与天然骨相似的结构和生物化学成分，是骨工程的理想支架材料。我们假设，微载体形式的脱钙骨基质有利于血管长入和骨再生后，在体内植入。本研究通过将脱钙骨基质支架填充在血管蒂周围的硅胶腔中，建立大鼠股动脉血管蒂骨工程模型，比较微载体脱钙骨基质和块状脱钙骨基质血管化骨再生的效率。结果表明，骨髓干细胞在体外培养过程中与微载体脱钙骨基质的粘附性较好，并产生较多的细胞外基质。在体内植入后，微载体脱钙骨基质与骨髓干细胞接种形成相对更多的骨组织比块脱钙骨基质对应物在三个月后的组织学检查。显微CT三维重建显示，微载体脱钙骨基质组的骨组织再生质量和数量均明显优于块状脱钙骨基质组（p < 0.05）。微计算机断层血管重建图像显示，微载体脱钙骨基质组的组织血管化程度明显高于块状脱钙骨基质组（p < 0.05）。抗CD31免疫组化染色和半定量分析也显示微载体脱钙骨基质组的新生骨血管化程度明显高于块状脱钙骨基质组（p < 0.05）。总之，微载体形式的脱钙骨基质是一种理想的骨再生支架，因为它具有骨诱导性和血管诱导性的优点，这是体内骨再生的两个要素。
Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.
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影响因子：
2.3
作者：
J. Roth
通讯作者：
J. Roth
影响因子：
8
作者：
Liu H;Slamovich EB;Webster TJ
通讯作者：
Webster TJ
影响因子：
3.1
作者：
Y. Shi;G. Kramer;A. Schröder;C. Kirkpatrick;A. Seekamp;H. Schmidt;S. Fuchs
通讯作者：
Y. Shi;G. Kramer;A. Schröder;C. Kirkpatrick;A. Seekamp;H. Schmidt;S. Fuchs
DOI：
10.1007/978-1-4471-5451-8_132
发表时间：
2014
期刊：
--
影响因子：
--
作者：
G. Kumar;B. Narayan
通讯作者：
G. Kumar;B. Narayan
影响因子：
2.1
作者：
R. Walton;E. Beahm;Liza C. Wu
通讯作者：
R. Walton;E. Beahm;Liza C. Wu