NMR visibility of deuterium-labeled liver glycogen in vivo.
NMR visibility of deuterium-labeled liver glycogen in vivo.
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DOI：
10.1002/mrm.28717
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发表时间：
2021-07
影响因子：
3.3
通讯作者：
de Graaf RA
中科院分区：
文献类型：
--
作者：
De Feyter HM;Thomas MA;Behar KL;de Graaf RA
氘代谢成像（DMI）联合[6,6 ' -2H2]-葡萄糖具有检测肝脏糖原合成的潜力。然而，与使用13C mrs的类似方法相比，[6,6 ' -2H2]-葡萄糖和[6,6 ' -2H2]-糖原在2H NMR光谱中的相似化学位移使得在体内进行明确的检测和分离变得困难。在这里，我们研究了2H标记的糖原的NMR可见性，以更好地了解在给予[6,6 ' -2H2]-葡萄糖后，它对肝脏中观察到的信号的潜在贡献。给小鼠提供含有2h标记葡萄糖的饮用水。在加入葡萄糖释放酶淀粉葡萄糖苷酶前后，对分离的肝糖原溶液进行了高分辨率核磁共振分析。由于2H标记的糖原的T2 （< 2 ms）非常短，其谱线宽度是13c标记的糖原的谱线宽度（T2 ~ 10 ms）的五倍多，因此使用2H核磁共振几乎无法在溶液中检测到2H标记的糖原。在体内条件下，2H标记的糖原无法用2H MRS(I)检测到，因此13C MRS是体内检测糖原的首选技术。
Deuterium Metabolic Imaging (DMI) combined with [6,6’-2H2]-glucose has the potential to detect glycogen synthesis in the liver. However, the similar chemical shifts of [6,6’-2H2]-glucose and [6,6’-2H2]-glycogen in the 2H NMR spectrum make unambiguous detection and separation difficult in vivo, in contrast to comparable approaches using 13C MRS. Here we investigate the NMR visibility of 2H-labeled glycogen to better understand its potential contribution to the observed signal in liver following administration of [6,6’-2H2]-glucose. Mice were provided drinking water containing 2H-labeled glucose. High resolution NMR analyses was performed of isolated liver glycogen in solution, before and after the addition of the glucose releasing enzyme amyloglucosidase. 2H-labeled glycogen was barely detectable in solution using 2H NMR, due to the very short T2 (< 2 ms) of 2H-labeled glycogen, giving a spectral line width that is more than five times as broad as that of 13C-labeled glycogen (T2 ~ 10 ms). 2H-labeled glycogen is not detectable with 2H MRS(I) under in vivo conditions, leaving 13C MRS as the preferred technique for in vivo detection of glycogen.
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影响因子：
158.5
作者：
SHULMAN, GI;ROTHMAN, DL;SHULMAN, RG
通讯作者：
SHULMAN, RG
影响因子：
56.9
作者：
ALGER, JR;SILLERUD, LO;HANLEY, PE
通讯作者：
HANLEY, PE
影响因子：
6.3
作者：
Lu, Ming;Zhu, Xiao-Hong;Chen, Wei
通讯作者：
Chen, Wei
影响因子：
10.6
作者：
PAULY, J;LEROUX, P;MACOVSKI, A
通讯作者：
MACOVSKI, A
影响因子：
13.6
作者：
De Feyter HM;Behar KL;Corbin ZA;Fulbright RK;Brown PB;McIntyre S;Nixon TW;Rothman DL;de Graaf RA
通讯作者：
de Graaf RA