Measurement of the localised plasmon penetration depth for gold nanoparticles using a non-invasive bio-stacking method.

Measurement of the localised plasmon penetration depth for gold nanoparticles using a non-invasive bio-stacking method.
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使用非侵入性生物堆积方法测量金纳米粒子的局部等离子体穿透深度。

DOI:
10.1039/c3cp50758k
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发表时间:
2013
期刊:
PCCP
影响因子:
--
通讯作者:
Read T
Read T
中科院分区:
--
文献类型:
--
作者:
Read T

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我们利用在金纳米颗粒和连续金表面上形成多达24个台阶的生物探针堆栈,通过仅涉及标准生物测定中的生物分子,以非侵入性方式表征等离子体场的穿透深度。在蛋白A/G功能化的金表面聚合了抗山羊兔IgG和抗兔IgG交替的生物探针堆栈。虽然保持了抗体表位的生物完整性,但等离子激元激发角或光散射的变化随着每一步的叠加都呈指数递减。所得动力学参数的指数衰减归因于穿透深度的变化,并且使用商业连续金表面等离子体共振表面校准步长为17.5±0.8 nm,与抗体的预期尺寸一致。测定了直径为90±13 nm的金球形纳米颗粒的穿透深度为93±10 nm。
We have used the formation of a bio-probe stack with up to 24 steps on gold nanoparticle and continuous gold surfaces to characterize the penetration depth of the plasmon field in a non-invasive manner by only involving biomolecules from standard bio-assays. An alternating anti-goat rabbit IgG and anti-rabbit IgG bio-probe stack is polymerized on protein A/G functionalized gold surfaces. The change in plasmon excitation angle or light scattering decreases exponentially with each stacking step although the bio-integrity of the antibody epitope is maintained. The exponential decay in the derived kinetic parameters is attributed to the change in the penetration depth and the step size is calibrated using a commercial continuous gold surface plasmon resonance surface to be 17.5 ± 0.8 nm, consistent with the expected dimension of the antibody. The penetration depth of the gold spherical nanoparticles of diameter 90 ± 13 nm is determined to be 93 ± 10 nm.
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发表时间: 2008-03
影响因子: 12.6
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