Bacterial "Virulence" Traits and Host Demographics Predict Escherichia coli Colonization Behaviors Within Households.
Bacterial "Virulence" Traits and Host Demographics Predict Escherichia coli Colonization Behaviors Within Households.
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DOI：
10.1093/ofid/ofaa495
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发表时间：
2020-11
影响因子：
4.2
通讯作者：
Johnson JR
中科院分区：
文献类型：
--
作者：
Fox TC;Clabots C;Porter SB;Bender T;Thuras P;Colpan A;Boettcher J;Johnson JR
尽管肠道定植先于大多数肠外大肠杆菌感染，但促进定植的因素尚不完全清楚。我们比较了E.大肠杆菌定殖模式与宿主和细菌性状。22名退伍军人临床E。大肠杆菌分离株及其46名人类和动物家庭成员进行了纵向粪便采样。不同的E.大肠杆菌菌株的系统发育背景，毒力基因，抗生素耐药性和定植行为进行了表征。对宿主和细菌性状进行统计评估，作为定植行为的预测因素。在139个独特的家庭粪便E。大肠杆菌菌株的定殖行为的单变量预测因子包括（i）宿主人口统计学，（ii）与指示临床分离株相匹配，和（iii）细菌特性（2个亚群，5个克隆谱系，18个毒力基因，和分子肠外致病性大肠杆菌。大肠杆菌状态）。定殖行为的多变量预测因子包括资深宿主、配偶宿主、匹配的指示临床分离株、α组F、ST 73、hlyD（α溶血素）、hlyF（变体溶血素）、H7 fliC（鞭毛变体）、vat（空泡毒素）和iha（粘附素-铁载体）。宿主人口统计学、多种细菌“毒力”特征以及与临床分离株预测的E.大肠杆菌粪便定植行为。因此，某些细菌特性可促进定植和致病性。未来针对这些特征的干预措施可能会阻止E。大肠杆菌感染的直接和破坏先行殖民。
Although intestinal colonization precedes most extraintestinal Escherichia coli infections, colonization-promoting factors are incompletely understood. We compared within-household E. coli colonization patterns with host and bacterial traits. Twenty-two veterans with a clinical E. coli isolate and their 46 human and animal household members underwent longitudinal fecal sampling. Distinct E. coli strains were characterized for phylogenetic background, virulence genes, antibiotic resistance, and colonization behaviors. Host and bacterial traits were assessed statistically as predictors of colonization behaviors. Among the 139 unique-by-household fecal E. coli strains, univariable predictors of colonization behavior included (i) host demographics, (ii) matching the index clinical isolate, and (iii) bacterial characteristics (2 phylogroups, 5 clonal lineages, 18 virulence genes, and molecular extraintestinal pathogenic E. coli status). Multivariable predictors of colonization behavior included veteran host, spouse host, matching the index clinical isolate, phylogroup F, ST73, hlyD (alpha hemolysin), hlyF (variant hemolysin), H7 fliC (flagellar variant), vat (vacuolating toxin), and iha (adhesin-siderophore). Host demographics, multiple bacterial “virulence” traits, and matching the index clinical isolate predicted E. coli fecal colonization behaviors. Thus, certain bacterial characteristics may promote both colonization and pathogenicity. Future interventions directed toward such traits might prevent E. coli infections both directly and by disrupting antecedent colonization.
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影响因子：
2.4
作者：
Huang IF;Lee WY;Wang JL;Hung CH;Hu HH;Hung WY;Hung YJ;Chen WC;Shen YT;Cheng MF
通讯作者：
Cheng MF
影响因子：
3.2
作者：
BRIKUN, I;SUZIEDELIS, K;BERG, DE
通讯作者：
BERG, DE
影响因子：
5.8
作者：
Moreno, Eva;Johnson, James R.;Andreu, Antonia
通讯作者：
Andreu, Antonia
影响因子：
9.4
作者：
Moreno, Eva;Andreu, Antonia;Prats, Guillem
通讯作者：
Prats, Guillem
影响因子：
4.2
作者：
Johnson JR;Davis G;Clabots C;Johnston BD;Porter S;DebRoy C;Pomputius W;Ender PT;Cooperstock M;Slater BS;Banerjee R;Miller S;Kisiela D;Sokurenko EV;Aziz M;Price LB
通讯作者：
Price LB