Genotyping by sequencing reveals contrasting patterns of population structure, ecologically mediated divergence, and long-distance dispersal in North American palms.
Genotyping by sequencing reveals contrasting patterns of population structure, ecologically mediated divergence, and long-distance dispersal in North American palms.
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DOI：
10.1002/ece3.4125
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发表时间：
2018-06
影响因子：
2.6
通讯作者：
Hoffman JI
中科院分区：
文献类型：
--
作者：
Klimova A;Ortega-Rubio A;Vendrami DLJ;Hoffman JI
比较研究可以为影响种群分化的过程提供强有力的见解，从而有助于阐明当代人口可能对环境变化做出反应的机制。此外，测序基因分型(GBS)等方法为解决物种和种群之间的遗传差异提供了前所未有的力量。因此，我们使用GBS来提供关于下加利福尼亚半岛上两个棕榈属的比较种群结构的全基因组视角，华盛顿属和布拉希亚属，这是一个高度景观和生态复杂性的地区。首先，我们使用系统发育分析来解决目前公认的五个物种在分类学上的不确定性。我们解析了三个主要分支，第一个对应于W.robusta和W.C.filifera，第二个对应于B.Branddegeei和B.C.Armata，第三个对应于Guadalupe岛的B.eedulis。专注于前两个分支，然后我们通过调查潜在的种群结构进行了更深入的研究。发现了显著的差异，GBS发现了四个不同的华盛顿种群，并确定了一套与温度有关的基因座，这与生态调节的差异一致。相比之下，个别山脉可以在布拉希亚解决，很少有基因座与环境变量有关，这意味着中性分歧的作用更突出。最后，在华盛顿发现了远距离扩散事件的证据，而不是布拉希亚，这与这些棕榈树的扩散机制的知识一致，包括人类中介扩散的可能性。总体而言，我们的研究展示了GBS的力量，并用比较的方法阐明了由多个效应器介导的明显不同的全基因组差异模式。
Comparative studies can provide powerful insights into processes that affect population divergence and thereby help to elucidate the mechanisms by which contemporary populations may respond to environmental change. Furthermore, approaches such as genotyping by sequencing (GBS) provide unprecedented power for resolving genetic differences among species and populations. We therefore used GBS to provide a genomewide perspective on the comparative population structure of two palm genera, Washingtonia and Brahea, on the Baja California peninsula, a region of high landscape and ecological complexity. First, we used phylogenetic analysis to address taxonomic uncertainties among five currently recognized species. We resolved three main clades, the first corresponding to W. robusta and W. filifera, the second to B. brandegeei and B. armata, and the third to B. edulis from Guadalupe Island. Focusing on the first two clades, we then delved deeper by investigating the underlying population structure. Striking differences were found, with GBS uncovering four distinct Washingtonia populations and identifying a suite of loci associated with temperature, consistent with ecologically mediated divergence. By contrast, individual mountain ranges could be resolved in Brahea and few loci were associated with environmental variables, implying a more prominent role of neutral divergence. Finally, evidence was found for long‐distance dispersal events in Washingtonia but not Brahea, in line with knowledge of the dispersal mechanisms of these palms including the possibility of human‐mediated dispersal. Overall, our study demonstrates the power of GBS together with a comparative approach to elucidate markedly different patterns of genomewide divergence mediated by multiple effectors.
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影响因子：
9.2
作者：
Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
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作者：
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