Regulation of tomato fruit elongation by transcription factor BZR1.7 through promotion of SUN gene expression.
Regulation of tomato fruit elongation by transcription factor BZR1.7 through promotion of SUN gene expression.
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DOI：
10.1093/hr/uhac121
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发表时间：
2022
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
果形是番茄的一个重要生物学性状，具有特殊的商品价值。SUN基因是番茄果实伸长的关键调控因子，但其转录调控的分子机制仍不清楚。在这里，一个独特的BZR 1样转录因子，BZR1.7，被确定为SUN基因启动子的反式作用因子，结合到启动子的保守E-box，以促进SUN基因的表达。BZR1.7在番茄中的过表达导致SUN基因的表达量增加，并形成细长的果实。通过基因编辑产生的BZR1.7敲除突变体的植物没有表现出可观察到的果实形状表型，这表明番茄中BZR 1样基因可能存在功能冗余。在番茄基因组中有7个BZR 1样基因，并且BZR1.5和BZR1.6的过表达导致了与在BZR1.7过表达系中观察到的相似的延长的果实表型，进一步支持了在番茄果实形状指定中BZR 1样基因的功能冗余的观点。显微镜下观察发现，在BZR 1. 7过表达株系中，果皮细胞层数减少。这些发现为BZR1.7促进SUN基因表达和调节番茄果实伸长的调控机制提供了新的见解。
Fruit shape is an important biological trait that is also of special commercial value in tomato. The SUN gene has been known as a key regulator of tomato fruit elongation for years, but the molecular mechanisms underlying its transcriptional regulation remain little understood. Here, a unique BZR1-like transcription factor, BZR1.7, was identified as a trans-acting factor of the SUN gene promoter that bound to the conserved E-box of the promoter to promote SUN gene expression. Overexpression of BZR1.7 in tomato led to elevated SUN gene expression and formation of elongated fruits. Plants of the BZR1.7 knockout mutant created by gene editing did not exhibit an observable fruit shape phenotype, suggesting possible functional redundancy of BZR1-like genes in tomato. There were seven BZR1-like genes in the tomato genome and overexpression of BZR1.5 and BZR1.6 led to elongated fruit phenotypes similar to those observed in the BZR1.7 overexpression lines, further supporting the notion of functional redundancy of BZR1-like genes in tomato fruit shape specification. Microscopic analysis revealed that there was a decreased number of cell layers in the fruit pericarp in the BZR1.7 overexpression lines. These findings offer new insights into the regulatory mechanism by which BZR1.7 promotes SUN gene expression and regulates fruit elongation in tomato.
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