SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1.
SlBES1 promotes tomato fruit softening through transcriptional inhibition of PMEU1.
复制标题
SlBES1通过抑制PMEU1转录促进番茄果实软化。
DOI：
10.1016/j.isci.2021.102926
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发表时间：
2021-08-20
期刊：
影响因子：
5.8
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Liu H;Liu L;Liang D;Zhang M;Jia C;Qi M;Liu Y;Shao Z;Meng F;Hu S;Yin Y;Li C;Wang Q
由硬度表示的果实软化决定了番茄制品的质地、运输性和货架期。然而，番茄果实硬度形成的调控机制知之甚少。在这里，我们报告的调节作用SlBES 1，油菜素类固醇激素信号的重要组成部分，在番茄果实软化。我们发现SlBES 1在番茄果实成熟和采后贮藏期间促进果实软化。RNA-seq分析表明，编码果胶甲酯酶的PMEU 1可能参与SlBES 1介导的软化。生化和免疫荧光检测表明，SlBES 1抑制PMEU 1相关的果胶脱甲酯化。进一步的分子和遗传证据证实，SlBES 1直接与PMEU 1的E-box结合，抑制其表达，导致果实软化。由CRISPR-Cas9产生的功能丧失的SlBES 1突变体在采后储存期间显示出更结实的果实和更长的货架期，而没有其他品质改变。总的来说，我们的研究结果表明，操纵SlBES 1调节硬度的潜力，而不会对视觉和营养品质产生负面影响。SlBES 1促进番茄果实软化而不影响营养品质SlBES 1抑制PMEU 1相关的果实果胶脱甲基化SlBES 1通过直接结合E盒抑制PMEU 1表达通过CRISPR-Cas9敲除SlBES 1增强果实硬度并延长货架期生物科学;分子生物学;植物生物学;植物发育
Fruit softening indicated by firmness determines the texture, transportability, and shelf life of tomato products. However, the regulatory mechanism underlying firmness formation in tomato fruit is poorly understood. Here, we report the regulatory role of SlBES1, an essential component of brassinosteroid hormone signaling, in tomato fruit softening. We found that SlBES1 promotes fruit softening during tomato fruit ripening and postharvest storage. RNA-seq analysis suggested that PMEU1, which encodes a pectin methylesterase, might participate in SlBES1-mediated softening. Biochemical and immunofluorescence assays indicated that SlBES1 inhibited PMEU1-related pectin de-methylesterification. Further molecular and genetic evidence verified that SlBES1 directly binds to the E-box of PMEU1 to repress its expression, leading to fruits softening. Loss-of-function SlBES1 mutant generated by CRISPR-Cas9 showed firmer fruits and longer shelf life during postharvest storage without other quality alteration. Collectively, our results indicated the potential of manipulating SlBES1 to regulate firmness without negative consequence on visual and nutrition quality. SlBES1 promotes tomato fruit softening without affecting nutritional quality SlBES1 inhibits PMEU1-related fruit pectin de-methylesterification SlBES1 represses PMEU1 expression through directly binding to the E-box Knockout of SlBES1 by CRISPR-Cas9 enhances fruit firmness and extends shelf life Biological sciences; Molecular biology; Plant biology; Plant development
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影响因子：
56.9
作者：
He, JX;Gendron, JM;Wang, ZY
通讯作者：
Wang, ZY
影响因子：
27.5
作者：
Ma, Xingliang;Zhang, Qunyu;Liu, Yao-Guang
通讯作者：
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番茄果实中病毒诱导的基因沉默。
影响因子：
7.2
作者：
Fu, DQ;Zhu, BZ;Luo, YB
通讯作者：
Luo, YB
影响因子：
7.2
作者：
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通讯作者：
Yin, Yanhai
影响因子：
7.4
作者：
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通讯作者：
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