Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea.
Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea.
复制标题
细胞质雄性不育相关orf220基因的线粒体靶向表达导致芥菜雄性不育
DOI：
10.1186/1471-2229-10-231
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发表时间：
2010-10-26
影响因子：
5.3
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Yang J;Liu X;Yang X;Zhang M
背景
线粒体基因组重排产生的新型嵌合开放阅读框架(ORF)被普遍认为是细胞质雄性不育(CMS)发生的原因之一。当CMS相关的ORF被表达并定位于线粒体时，CMS相关的ORF与CMS的发生既有正相关，也有负相关。有些ORF会导致男性不育或半不育，而有些则不会。目前关于线粒体因子如何调节与男性不育有关的核基因的表达知之甚少。本研究的目的是研究新从细胞质雄性不育芥菜中分离到的一个与CMS相关的候选orf220基因的生物学功能，并揭示线粒体逆行调控核基因表达与雄性不育的关系。
结果
结果表明，ORF220蛋白可以通过F1-ATPaseβ亚单位(ATP2-1)的线粒体靶向序列被引导到线粒体。转基因茎用芥菜植株表达了含有orf220基因和F1-β酶亚基线粒体靶向序列(ATP2-1)的嵌合基因。转基因植株是雄性不育的，大多数不能产生花粉，有些只能产生不旺盛的花粉。转基因茎用芥菜植株也表现出与CMS茎用芥菜相同的花发育异常。寡核苷酸芯片分析结果显示，一些与线粒体能量代谢相关的基因表达下调，表明转基因茎芥菜的线粒体功能减弱。在转基因茎用芥菜中，一些与花粉发育相关的基因表达下调，一些转录因子基因的表达也发生了变化。
结论
这项工作进一步加深了我们对CMS相关orf220基因线粒体靶向表达如何通过逆行调节芥菜核基因表达而导致雄性不育的理解。
Background
The novel chimeric open reading frame (orf) resulting from the rearrangement of a mitochondrial genome is generally thought to be a causal factor in the occurrence of cytoplasmic male sterility (CMS). Both positive and negative correlations have been found between CMS-associated orfs and the occurrence of CMS when CMS-associated orfs were expressed and targeted at mitochondria. Some orfs cause male sterility or semi-sterility, while some do not. Little is currently known about how mitochondrial factor regulates the expression of the nuclear genes involved in male sterility. The purpose of this study was to investigate the biological function of a candidate CMS-associated orf220 gene, newly isolated from cytoplasmic male-sterile stem mustard, and show how mitochondrial retrograde regulated nuclear gene expression is related to male sterility.
Results
It was shown that the ORF220 protein can be guided to the mitochondria using the mitochondrial-targeting sequence of the β subunit of F1-ATPase (atp2-1). Transgenic stem mustard plants expressed the chimeric gene containing the orf220 gene and a mitochondrial-targeting sequence of the β subunit of F1-ATPase (atp2-1). Transgenic plants were male-sterile, most being unable to produce pollen while some could only produce non-vigorous pollen. The transgenic stem mustard plants also showed aberrant floral development identical to that observed in the CMS stem mustard phenotype. Results obtained from oligooarray analysis showed that some genes related to mitochondrial energy metabolism were down-regulated, indicating a weakening of mitochondrial function in transgenic stem mustard. Some genes related to pollen development were shown to be down-regulated in transgenic stem mustard and the expression of some transcription factor genes was also altered.
Conclusion
The work presented furthers our understanding of how the mitochondrially-targeted expression of CMS-associated orf220 gene causes male sterility through retrograde regulation of nuclear gene expression in Brassica juncea.
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通讯作者：
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