Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions.
Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions.
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DOI：
10.1186/1746-4811-8-20
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发表时间：
2012-06-21
期刊：
影响因子：
5.1
通讯作者：
Scholes J
中科院分区：
文献类型：
--
作者：
Runo S;Macharia S;Alakonya A;Machuka J;Sinha N;Scholes J
独脚金属有毒的根部半寄生杂草，会削弱撒哈拉以南非洲 (SSA) 的谷物产量。独脚金的控制选择是有限的，开发独脚金抗性作物种质被认为是最好和最可持续的控制措施。通过非基因改造（GM）方法（例如通过利用天然抗性或通过转基因方法）改善独脚金抗性种质的努力受到关于支撑宿主-寄生虫关联的生物过程的有限信息的限制。此外，转基因方法因缺乏可引入宿主的候选抗性基因和验证基因功能的稳健转化方法而受到阻碍。事实上，独脚金（世界上种植最多的谷物）的大多数宿主都难以进行基因转化。在玉米中，现有的转化和再生方案繁琐、冗长、基因型特异性强、转化效率低。我们使用携带报告基因构建体绿色荧光蛋白（GFP）的发根农杆菌菌株K599来产生转基因复合玉米植物，并用寄生植物独脚金进行攻击。百分之八十五的玉米植物产生了表达 GFP 的转基因毛状根。与其他物种产生的大多数毛状根一致，转化的玉米根表现出毛状根表型，这是发根农杆菌介导的转化的标志。由A. rhizogenes 转化产生的转基因毛状根很容易被S. hermonthica 感染。从转基因或野生型根中回收的 S. hermonthica 个体的数量和大小没有显着差异。这种快速、高通量的转化技术将增进我们对寄生植物与宿主相互作用中基因功能的理解。
Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarded as the best and most sustainable control measure. Efforts to improve germplasm for Striga resistance by a non-Genetic Modification (GM) approach, for example by exploiting natural resistance, or by a GM approach are constrained by limited information on the biological processes underpinning host-parasite associations. Additionaly, a GM approach is stymied by lack of availability of candidate resistance genes for introduction into hosts and robust transformation methods to validate gene functions. Indeed, a majority of Striga hosts, the world’s most cultivated cereals, are recalcitrant to genetic transformation. In maize, the existing protocols for transformation and regeneration are tedious, lengthy, and highly genotype-specific with low efficiency of transformation. We used Agrobacterium rhizogenes strain K599 carrying a reporter gene construct, Green Fluorescent Protein (GFP), to generate transgenic composite maize plants that were challenged with the parasitic plant Striga hermonthica. Eighty five percent of maize plants produced transgenic hairy roots expressing GFP. Consistent with most hairy roots produced in other species, transformed maize roots exhibited a hairy root phenotype, the hallmark of A. rhizogenes mediated transformation. Transgenic hairy roots resulting from A. rhizogenes transformation were readily infected by S. hermonthica. There were no significant differences in the number and size of S. hermonthica individuals recovered from either transgenic or wild type roots. This rapid, high throughput, transformation technique will advance our understanding of gene function in parasitic plant-host interactions.
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发根农杆菌介导的超级根衍生百脉根植物的转化：功能基因组学的一个有价值的工具
影响因子：
5.3
作者：
Jian B;Hou W;Wu C;Liu B;Liu W;Song S;Bi Y;Han T
通讯作者：
Han T
影响因子：
14.8
作者：
Ishida, Yuji;Hiei, Yukoh;Komari, Toshihiko
通讯作者：
Komari, Toshihiko
影响因子：
6.2
作者：
Bandyopadhyay, Maumita;Jha, Sumita;Tepfer, David
通讯作者：
Tepfer, David
影响因子：
3.5
作者：
DIOUF, D;GHERBI, H;BOGUSZ, D
通讯作者：
BOGUSZ, D
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F