The microbiome of the middle meatus in healthy adults.
The microbiome of the middle meatus in healthy adults.
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DOI：
10.1371/journal.pone.0085507
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Frank DN
中科院分区：
文献类型：
--
作者：
Ramakrishnan VR;Feazel LM;Gitomer SA;Ir D;Robertson CE;Frank DN
鼻炎和鼻窦炎是多因素疾病过程，其中细菌可能在感染或刺激炎症过程中发挥作用。历史上，鼻窦炎一直是用基于培养的技术进行研究的，这些技术涉及疾病状态中的几种常见病原体。最近，美国国立卫生研究院人类微生物组项目检查了一些可接近的身体部位的微生物组，并证明了健康和患病患者之间的差异。最近基于DNA的鼻窦研究表明，健康的鼻窦并不像以前认为的那样是无菌的，但正常的鼻窦微生物组尚未得到彻底检查。中鼻道拭子样本采集自28例连续患者，这些患者没有鼻窦炎的体征或症状。使用定量PCR和16S rRNA焦磷酸测序评估这些标本中的细菌定植。所有受试者中鼻道细菌定植均呈阳性。金黄色葡萄球菌、表皮葡萄球菌和痤疮丙酸杆菌是检出的最普遍和最丰富的微生物。在正常状态下，鼻窦腔中存在丰富多样的细菌聚集体，包括通常在鼻咽中发现的机会致病菌。这项工作有助于建立一个基线，以了解鼻窦微生物组如何影响上呼吸道疾病。
Rhinitis and rhinosinusitis are multifactorial disease processes in which bacteria may play a role either in infection or stimulation of the inflammatory process. Rhinosinusitis has been historically studied with culture-based techniques, which have implicated several common pathogens in disease states. More recently, the NIH Human Microbiome Project has examined the microbiome at a number of accessible body sites, and demonstrated differences among healthy and diseased patients. Recent DNA-based sinus studies have suggested that healthy sinuses are not sterile, as was previously believed, but the normal sinonasal microbiome has yet to be thoroughly examined. Middle meatus swab specimens were collected from 28 consecutive patients presenting with no signs or symptoms of rhinosinusitis. Bacterial colonization was assessed in these specimens using quantitative PCR and 16S rRNA pyrosequencing. All subjects were positive for bacterial colonization of the middle meatus. Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acnes were the most prevalent and abundant microorganisms detected. Rich and diverse bacterial assemblages are present in the sinonasal cavity in the normal state, including opportunistic pathogens typically found in the nasopharynx. This work helps establish a baseline for understanding how the sinonasal microbiome may impact diseases of the upper airways.
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影响因子：
3.4
作者：
GLIKLICH, RE;METSON, R
通讯作者：
METSON, R
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
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DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
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Honda K
DOI：
10.1016/j.jaci.2010.10.048
发表时间：
2011-02
期刊：
The Journal of allergy and clinical immunology
影响因子：
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作者：
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通讯作者：
National Heart, Lung, and Blood Institute's Asthma Clinical Research Network
影响因子：
158.5
作者：
Bisgaard, Hans;Hermansen, Mette Northman;Pipper, Christian Bressen
通讯作者：
Pipper, Christian Bressen