Conservation of replication timing reveals global and local regulation of replication origin activity.
Conservation of replication timing reveals global and local regulation of replication origin activity.
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DOI：
10.1101/gr.139477.112
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发表时间：
2012-10
期刊：
影响因子：
7
通讯作者：
Nieduszynski CA
中科院分区：
文献类型：
--
作者：
Müller CA;Nieduszynski CA
DNA复制从称为复制起点的定义位置开始;一些起点高度活跃，而另一些则处于休眠状态，很少使用。起源也在其激活时间上不同，导致特定的基因组区域在特征时间和定义的时间顺序复制。在这里，我们报告了四种芽殖酵母基因组复制的比较：酿酒酵母，S。paradoxus，S. arboricolus和S. bayanus首先，我们发现，活跃的起源的位置主要是物种之间的保守，而休眠起源的保守性很差。其次，我们为这些物种中的每一个生成了全基因组复制谱，并发现基因组复制的时间顺序是高度保守的。因此，活性起源不仅在位置上是保守的，而且在激活时间上也是保守的。这些保守的起源中只有少数显示出这些物种之间激活时间的差异。为了深入了解起源激活时间的调节机制，我们生成了S。酿酒酵母/S. bayanus杂交菌株，并发现既有本地和全球的起源功能调节。
DNA replication initiates from defined locations called replication origins; some origins are highly active, whereas others are dormant and rarely used. Origins also differ in their activation time, resulting in particular genomic regions replicating at characteristic times and in a defined temporal order. Here we report the comparison of genome replication in four budding yeast species: Saccharomyces cerevisiae, S. paradoxus, S. arboricolus, and S. bayanus. First, we find that the locations of active origins are predominantly conserved between species, whereas dormant origins are poorly conserved. Second, we generated genome-wide replication profiles for each of these species and discovered that the temporal order of genome replication is highly conserved. Therefore, active origins are not only conserved in location, but also in activation time. Only a minority of these conserved origins show differences in activation time between these species. To gain insight as to the mechanisms by which origin activation time is regulated we generated replication profiles for a S. cerevisiae/S. bayanus hybrid strain and find that there are both local and global regulators of origin function.
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影响因子：
21.3
作者：
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