Diversity in insect axis formation: two orthodenticle genes and hunchback act in anterior patterning and influence dorsoventral organization in the honeybee (Apis mellifera)

Diversity in insect axis formation: two orthodenticle genes and hunchback act in anterior patterning and influence dorsoventral organization in the honeybee (Apis mellifera)
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昆虫轴形成的多样性:两个正牙齿基因和驼背在前部模式中起作用并影响蜜蜂(Apis mellifera)的背腹组织

DOI:
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发表时间:
2011
期刊:
影响因子:
4.6
通讯作者:
P. Dearden
P. Dearden
中科院分区:
生物学2区
文献类型:
--
作者:
M. Wilson;P. Dearden

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轴的形成是发育的关键步骤,但研究表明,参与昆虫轴形成的基因进化相对较快。直齿基因在几种昆虫的母体前部模式中具有保守的作用,通常具有驼背。我们发现,两个orthodenticle基因,otd1和otd2,驼背作为母亲的前模式基因在蜜蜂(意大利蜜蜂),但与其他昆虫不同,作用模式的大部分前后轴。这些基因调控前间隙、中央间隙和后间隙基因的表达结构域,并可能直接调控前间隙基因巨人。我们表明otd 1和驼背也影响背腹图案通过调节zerknült(禅),因为他们在赤拟谷盗,但禅不调节蜜蜂间隙基因的表达。这表明前后和背腹模式之间的相互作用是全变态昆虫的祖先。蜜蜂轴的形成,和保守的前图案化基因orthodenticle的功能,显示出独特的字符,表明,即使当保守的基因图案的轴,它们的监管相互作用不同的昆虫的订单,与相对较快的进化轴形成途径。
Axis formation is a key step in development, but studies indicate that genes involved in insect axis formation are relatively fast evolving. Orthodenticle genes have conserved roles, often with hunchback, in maternal anterior patterning in several insect species. We show that two orthodenticle genes, otd1 and otd2, and hunchback act as maternal anterior patterning genes in the honeybee (Apis mellifera) but, unlike other insects, act to pattern the majority of the anteroposterior axis. These genes regulate the expression domains of anterior, central and posterior gap genes and may directly regulate the anterior gap gene giant. We show otd1 and hunchback also influence dorsoventral patterning by regulating zerknült (zen) as they do in Tribolium, but that zen does not regulate the expression of honeybee gap genes. This suggests that interactions between anteroposterior and dorsal-ventral patterning are ancestral in holometabolous insects. Honeybee axis formation, and the function of the conserved anterior patterning gene orthodenticle, displays unique characters that indicate that, even when conserved genes pattern the axis, their regulatory interactions differ within orders of insects, consistent with relatively fast evolution in axis formation pathways.
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