Monolithic column based on a poly(glycidyl methacrylate-co-4-vinylphenylboronic acid-co-ethylene dimethacrylate) copolymer for capillary liquid chromatography of small molecules and proteins.
Monolithic column based on a poly(glycidyl methacrylate-co-4-vinylphenylboronic acid-co-ethylene dimethacrylate) copolymer for capillary liquid chromatography of small molecules and proteins.
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基于聚（甲基丙烯酸缩水甘油酯-共-4-乙烯基苯基硼酸-共-二甲基丙烯酸乙二酯）共聚物的整体柱，用于小分子和蛋白质的毛细管液相色谱。
DOI：
10.1016/j.chroma.2012.02.056
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发表时间：
2012-07
影响因子：
4.1
通讯作者：
Zian Lin*, Hui Huang, Xiaobo Sun, Yao Lin, Lan Zha
中科院分区：
文献类型：
--
作者：
Zian Lin*, Hui Huang, Xiaobo Sun, Yao Lin, Lan Zha
在100μm内径下合成了一种具有反相、亲水和阳离子交换三种作用模式的新型聚合物整体柱。熔融石英毛细管通过原位聚合程序。以甲基丙烯酸缩水甘油酯（GMA）和4-乙烯基苯基硼酸（VPBA）为双官能度单体，二甲基丙烯酸乙二醇酯（EDMA）为交联剂，1，4-丁二醇（BDO）和二甘醇（DEG）为二元致孔剂，偶氮二异丁腈（AIBN）为引发剂，制备了一种新型的纳米复合材料。所得的聚（GMA-co-VPBA-co-EDMA）整体显示出相对均匀的整体结构、良好的渗透性和机械稳定性。采用不同比例的单体和致孔剂优化整体柱的性能。通过对一系列中性溶质、电荷溶质、酚类和苯胺类化合物的分离来评价柱的性能。与poly（GMA-co-EDMA）整体柱相比，在相同的色谱条件下，该整体柱在疏水性、亲水性以及阳离子交换作用方面表现出更灵活的选择性调节。在0.265mm/s的线速度下，苯系物的柱效可达70，000 -102，000理论塔板/m。保留时间的运行间、色谱柱间和批间重复性均小于8.23%。此外，该整体柱还用于生物碱和蛋白质的高效分离，以展示其在生物分子分离中的潜力。
A new polymer monolith with three modes of reverse-phase, hydrophilic and cation-exchange interaction was synthesized in 100μm i.d. fused-silica capillary by in situ polymerization procedure. The pre-polymerization mixture consisted of glycidyl methacrylate (GMA) and 4-vinylphenylboronic acid (VPBA) as bifunctional monomers, ethylene dimethacrylate (EDMA) as crosslinker, 1,4-butanediol (BDO) and diethylene glycol (DEG) as binary porogenic solvents, and azobisisobutyronitrile (AIBN) as initiator. The resulting poly(GMA-co-VPBA-co-EDMA) monolith showed a relatively homogeneous monolithic structure, good permeability and mechanical stability. Different ratios of monomers and porogens were used for optimizing the properties of monolithic column. The column performance was assessed by the separation of a series of neutral solutes, charge solutes, phenols and anilines. Compared with poly(GMA-co-EDMA) monolith, the proposed monolith exhibited more flexible adjustment of selectivity in terms of hydrophobic, hydrophilic, as well as cation-exchange interaction in the same chromatographic conditions. High column efficiencies for benzene derivatives with 70,000–102,000theoreticalplates/m could be obtained at a linear velocity of 0.265mm/s. The run-to-run, column-to-column, and batch-to-batch repeatabilities of the retention times were less than 8.23%. Additionally, the purposed monolith was also applied to efficient separation of alkaloids and proteins for demonstrating its potential in biomolecule separation.
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影响因子：
4.1
作者：
Frankovic, Vida;Podgornik, Ales;Strancar, Ales
通讯作者：
Strancar, Ales
DOI：
10.1016/j.chroma.2006.07.029
发表时间：
2006-10
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Gengliang Yang;Haiyan Liu;Yihua Zhang;Sumin Wang;Junfa Yin;Boying Yin;Yi Chen
通讯作者：
Gengliang Yang;Haiyan Liu;Yihua Zhang;Sumin Wang;Junfa Yin;Boying Yin;Yi Chen
DOI：
10.1016/j.chroma.2007.10.068
发表时间：
2008-04
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Yu-Ping Zhang;X. Ye;Mengkui Tian;Ling‐bo Qu;Seong-Ho Choi;A. Gopalan;Kwang-Pil Lee
通讯作者：
Yu-Ping Zhang;X. Ye;Mengkui Tian;Ling‐bo Qu;Seong-Ho Choi;A. Gopalan;Kwang-Pil Lee
DOI：
10.1016/s0021-9673(02)00072-9
发表时间：
2002-04
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Hanfa Zou;Xiaodong Huang;M. Ye;Q. Luo
通讯作者：
Hanfa Zou;Xiaodong Huang;M. Ye;Q. Luo
用于毛细管液相色谱选择性捕获糖蛋白的苯硼酸亲和整体料的制备和评价
影响因子：
4.2
作者：
Lin, Zi An;Pang, Ji Lei;Chen, Guo Nan
通讯作者：
Chen, Guo Nan