Spatiotemporal expression of histone acetyltransferases, p300 and CBP, in developing embryonic hearts.
Spatiotemporal expression of histone acetyltransferases, p300 and CBP, in developing embryonic hearts.
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DOI：
10.1186/1423-0127-16-24
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发表时间：
2009-02-23
影响因子：
11
通讯作者：
Tian J
中科院分区：
文献类型：
--
作者：
Chen G;Zhu J;Lv T;Wu G;Sun H;Huang X;Tian J
组蛋白乙酰转移酶（HATs）、p300和cAMP反应元件结合蛋白（CREB）结合蛋白（CBP）是两种结构相关的转录共激活因子，可激活许多真核生物基因的表达，这些基因参与细胞生长和信号转导、肌肉分化和胚胎发生。然而，这些蛋白是否在小鼠心脏发生中发挥重要的不同作用尚不清楚。本研究采用免疫组织化学和RT-PCR技术，研究了正常心脏发育过程中胚胎和成年小鼠心脏中这两种hat的蛋白分布和mRNA表达。免疫组织化学实验的数据显示p300广泛存在于从胚胎期到成年期的几乎每个心脏区域。然而，E7.5天胚胎心脏未检测到CBP的表达。CBP的表达出现在晚期，且CBP的分布小于p300。在E10.5后发育的心脏中，p300和CBP mRNA的表达水平在E10.5 d达到峰值，之后逐渐降低。这些结果表明p300和CBP都与胚胎心脏发育有关。这两种酶在小鼠心脏发育过程中的动态表达模式表明它们可能在心脏发育中起重要作用。然而，这两种酶在心脏发育过程中的时空表达模式存在差异。p300的表达更早、更显著，提示p300可能在胚胎心脏发育过程中发挥更重要的作用，特别是在心脏前体细胞诱导和室间隔形成过程中。
Histone acetyltransferases (HATs), p300 and cAMP response element binding protein (CREB)-binding protein (CBP) are two structurally related transcriptional co-activators that activate expression of many eukaryotic genes involved in cellular growth and signaling, muscle differentiation and embryogenesis. However, whether these proteins play important and different roles in mouse cardiogenesis is not clear. Here, we investigate the protein distributions and mRNA expression of the two HATs in embryonic and adult mouse heart during normal heart development by using immunohistochemical and RT-PCR techniques. The data from immunohistochemical experiments revealed that p300 was extensively present in nearly every region of the hearts from embryonic stages to the adulthood. However, no CBP expression was detected in embryonic hearts at day E7.5. CBP expression appeared at the later stages, and the distribution of CBP was less than that of p300. In the developmental hearts after E10.5, both for p300 and CBP, the mRNA expression levels reached a peak on day E10.5, and then were gradually decreased afterwards. These results reveal that both p300 and CBP are related to embryonic heart development. The dynamic expression patterns of these two enzymes during mouse heart development indicate that they may play an important role on heart development. However, there is a difference in spatiotemporal expression patterns between these two enzymes during heart development. The expression of p300 is earlier and more predominate, suggesting that p300 may play a more important role in embryonic heart development especially during cardiac precursor cell induction and interventricular septum formation.
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影响因子：
64.8
作者：
PETRIJ, F;GILES, RH;BREUNING, MH
通讯作者：
BREUNING, MH
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作者：
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影响因子：
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作者：
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通讯作者：
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