Efficacy of Sida pilosa Retz aqueous extract against Schistosoma mansoni - induced granulomatous inflammation in the liver and the intestine of mice: histomorphometry and gastrointestinal motility evaluation.
Efficacy of Sida pilosa Retz aqueous extract against Schistosoma mansoni - induced granulomatous inflammation in the liver and the intestine of mice: histomorphometry and gastrointestinal motility evaluation.
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SIDA PILOSA RETZ水提取物的功效针对曼氏血吸虫诱导的肝脏炎症和小鼠肠中的肉芽肿性炎症：组织形态计量学和胃肠道运动评估。
DOI：
10.1186/s12906-018-2318-2
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发表时间：
2018-09-06
影响因子：
--
通讯作者：
Kamtchouing P
中科院分区：
文献类型：
--
作者：
Jatsa HB;Femoe UM;Njiaza J;Tombe Tombe DS;Mbolang LN;Nkondo ET;Tchuente LT;Dimo T;Kamtchouing P
毛缕三叶浸膏是治疗肠蠕虫病的经验用药。已有研究表明，芦笋水提物（SpAE）对曼氏血吸虫感染具有杀血吸虫、抗氧化、抗炎和抗纤维化作用。本研究采用组织形态学法评价SpAE对小鼠肝、肠肉芽肿性炎症的影响；对胃肠运动也有影响。为了研究SpAE对肝脏和肠道组织形态学及胃肠运动的影响，我们以200 mg/kg / s的剂量给药mansoni感染小鼠4周。以吡喹酮为对照药。在进行牺牲之前，用3%炭粉对一批小鼠进行胃肠运输评估。在另一批小鼠牺牲后，我们对肝脏和回肠进行了组织学和形态计量学分析。我们测量了以下指标：总蛋白、转氨酶、丙二醛、亚硝酸盐、超氧化物歧化酶、过氧化氢酶和还原性谷胱甘肽。在没有或存在药物阻滞剂的情况下，评估了SpAE（4、8、16和32 mg/mL）对回肠收缩活性的影响。SpAE可显著减少肝脾肿大和肠肿大。肝肉芽肿数量减少52.82%，肠肉芽肿数量减少52.79%，肝肉芽肿体积减少48.76%。SpAE也降低了回肠肌层厚度（p < 0.001）。经SpAE治疗后，总蛋白、转氨酶、丙二醛、亚硝酸盐、超氧化物歧化酶、过氧化氢酶、还原性谷胱甘肽水平均恢复正常。SpAE治疗后也记录了胃肠运输的正常化。SpAE对肠道运动的影响是通过细胞内和细胞外钙动员介导的。我们的研究结果提供了证据，表明SpAE改善了肝脏和肠道中由曼氏梭菌引起的肉芽肿性炎症，并重建了正常的胃肠道运输。SpAE可用于开发抗曼氏链球菌感染的替代药物。
The macerate of Sida pilosa aerial parts is used empirically for the treatment of intestinal helminthiasis. Previous studies have shown that Sida pilosa aqueous extract (SpAE) has schistosomicidal, antioxidant, anti-inflammatory and anti-fibrotic activities in Schistosoma mansoni infection. This study was designed to evaluate the effect of SpAE on the granulomatous inflammation induced by S. mansoni in the liver and the intestine of mice by histomorphometry; as well as on the gastrointestinal motility. To study the effect of SpAE on the liver and intestine histomorphometry and on the gastrointestinal motility, SpAE was administered at 200 mg/kg per os to S. mansoni-infected mice for 4 weeks. Praziquantel was used as reference drug. Prior to carrying out sacrifice, a batch of mice was subjected to gastrointestinal transit evaluation with 3% charcoal meal. After sacrifying another batch of mice, we performed histological and morphometric analyses of the liver and the ileum. We measured the following: total proteins, transaminases, malondialdehyde, nitrites, superoxide dismutase, catalase and reduced glutathione. The effect of SpAE (4, 8, 16 and 32 mg/mL) on the ileum contractile activity was evaluated either in the absence or in the presence of pharmacological blockers. SpAE induced a significant reduction of hepatosplenomegaly and intestine enlargement. The number of granulomas was reduced by 52.82% in the liver and 52.79% in the intestine, whereas the volume of hepatic granulomas decreased by 48.76% after SpAE treatment. SpAE also reduced (p < 0.001) the ileal muscular layer thickness. The levels of total proteins, transaminases, malondialdehyde, nitrites, superoxide dismutase, catalase and reduced glutathione were restored after treatment of infected mice with SpAE. A normalization of the gastrointestinal transit was also recorded after SpAE treatment. The effect of SpAE on intestinal motility was mediated via intracellular and extracellular calcium mobilization. Our findings provide evidence that SpAE improves granulomatous inflammation induced by S. mansoni both in the liver and in the intestine, as well as it re-establishes normal gastrointestinal transit. SpAE may be used for the development of alternative medicine against S. mansoni infection.
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影响因子：
4
作者：
De Man, JG;Chatterjee, S;Pelckmans, PA
通讯作者：
Pelckmans, PA
影响因子：
--
作者：
Kim, Young Chul;Hayase, Masa;Suzuki, Hikaru
通讯作者：
Suzuki, Hikaru
影响因子：
5.4
作者：
LUTTERODT, GD
通讯作者：
LUTTERODT, GD
影响因子：
9.3
作者：
Lutterodt, GD
通讯作者：
Lutterodt, GD
影响因子：
6.7
作者：
Dkhil MA
通讯作者：
Dkhil MA