Remodelling of the Vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments.
Remodelling of the Vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments.
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DOI：
10.1111/j.1365-2958.2010.07476.x
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发表时间：
2011-02
影响因子：
3.6
通讯作者：
Trent MS
中科院分区：
文献类型：
--
作者：
Giles DK;Hankins JV;Guan Z;Trent MS
革兰氏阴性细菌霍乱弧菌引起严重的公共卫生问题，造成急性肠道感染，每年困扰数百万人。已显示胆汁影响霍乱弧菌的运动性以及毒力因子表达和外膜蛋白产生。本研究检查了胆汁对V. cholesterol磷脂的影响。胆汁暴露导致胆固醇弧菌的磷脂谱发生显著改变，但对其他肠道病原体没有影响。这些变化包括心磷脂的定量增加和迁移差异，磷脂酰甘油和磷脂酰乙醇胺的减少，以及确定为溶血磷脂酰乙醇胺的未知磷脂的戏剧性出现。胆汁的主要成分不是观察到的变化的原因，但长链多不饱和脂肪酸（胆汁的次要成分）被证明掺入胆固醇弧菌的磷脂中。虽然胆汁诱导的磷脂谱是独立的霍乱弧菌毒力级联，我们确定了另一个相关的环境中，霍乱弧菌同化独特的脂肪酸进入其膜磷脂-海洋沉积物。我们的研究结果表明，弧菌物种具有独特的机制，赋予的能力，采取了更广泛的外源性脂肪酸比其他肠道细菌。
The Gram-negative bacteria Vibrio cholerae poses significant public health concerns by causing an acute intestinal infection afflicting millions of people each year. V. cholerae motility, as well as virulence factor expression and outer membrane protein production, have been shown to be affected by bile. The current study examines the effects of bile on V. cholerae phospholipids. Bile exposure caused significant alterations to the phospholipid profile of V. cholerae but not of other enteric pathogens. These changes consisted of a quantitative increase and migratory difference in cardiolipin, decreases in phosphatidylglycerol and phosphatidylethanolamine, and the dramatic appearance of an unknown phospholipid determined to be lyso-phosphatidylethanolamine. Major components of bile were not responsible for the observed changes, but long chain polyunsaturated fatty acids, which are minor components of bile, were shown to be incorporated into phospholipids of V. cholerae. Although the bile-induced phospholipid profile was independent of the V. cholerae virulence cascade, we identified another relevant environment in which V. cholerae assimilates unique fatty acids into its membrane phospholipids—marine sediment. Our results suggest that Vibrio species possess unique machinery conferring the ability to take up a wider range of exogenous fatty acids than other enteric bacteria.
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影响因子：
9.4
作者：
IWANAGA, M;YAMAMOTO, K
通讯作者：
YAMAMOTO, K
影响因子：
3.2
作者：
Chatterjee, A;Chaudhuri, S;Chowdhury, R
通讯作者：
Chowdhury, R
DOI：
10.3181/00379727-104-25780
发表时间：
1960-01-01
影响因子：
--
作者：
BLOMSTRAND, R;EKDAHL, P
通讯作者：
EKDAHL, P
影响因子：
56.9
作者：
Colwell, RR
通讯作者：
Colwell, RR
影响因子：
56.9
作者：
Hung, DT;Shakhnovich, EA;Mekalanos, JJ
通讯作者：
Mekalanos, JJ