Microfluidic aqueous two phase system for leukocyte concentration from whole blood.
Microfluidic aqueous two phase system for leukocyte concentration from whole blood.
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DOI：
10.1007/s10544-008-9238-8
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发表时间：
2009-04
影响因子：
2.8
通讯作者：
Walker GM
中科院分区：
文献类型：
--
作者：
Soohoo JR;Walker GM
使用微流体水性两相系统 (μATPS) 浓缩全血样本中的白细胞。将全血同时暴露于聚乙二醇 (PEG) 和葡聚糖 (Dex) 相流，并根据细胞对流的不同亲和力进行分配。微流体装置的层流特性用于在聚合物流之间创建零、一和两个稳定界面。评估了三种不同模式的三种聚合物流在浓缩白细胞方面的有效性：不混溶的 PEG-PEG-Dex、不混溶的 Dex-PEG-Dex 和混溶的 PEG-PBS-Dex。最有效的配置是 Dex-PEG-Dex 流模式，与未浓缩的血液相比，其平均白细胞与红细胞的比率增加了 9.13 倍。
Leukocytes from a whole blood sample were concentrated using a microfluidic aqueous two phase system (μATPS). Whole blood was simultaneously exposed to polyethylene glycol (PEG) and dextran (Dex) phase streams and cells were partitioned based on their differential affinity for the streams. The laminar flow characteristic of microfluidic devices was used to create zero, one, and two stable interfaces between the polymer streams. Three different patterns of three polymer streams each were evaluated for their effectiveness in concentrating leukocytes: immiscible PEG-PEG-Dex, immiscible Dex-PEG-Dex, and miscible PEG-PBS-Dex. The most effective configuration was the Dex-PEG-Dex stream pattern which on average increased the ratio of leukocytes to erythrocytes by a factor of 9.13 over unconcentrated blood.
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影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC
影响因子：
4.1
作者：
PINILLA, M;DELAFUENTE, J;LUQUE, J
通讯作者：
LUQUE, J
影响因子：
2.2
作者：
LUNDAHL, J;HALLDEN, G;HED, J
通讯作者：
HED, J
影响因子：
6.1
作者：
Meagher, Robert J.;Light, Yooli K.;Singh, Anup K.
通讯作者：
Singh, Anup K.
影响因子：
7.4
作者：
Sethu, P;Anahtar, M;Toner, M
通讯作者：
Toner, M