Hierarchical microtubule organization controls axon caliber and transport and determines synaptic structure and stability.
Hierarchical microtubule organization controls axon caliber and transport and determines synaptic structure and stability.
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DOI：
10.1016/j.devcel.2015.02.003
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发表时间：
2015-04-06
影响因子：
11.8
通讯作者：
Pielage J
中科院分区：
文献类型：
--
作者：
Stephan R;Goellner B;Moreno E;Frank CA;Hugenschmidt T;Genoud C;Aberle H;Pielage J
The dimensions of axons and synaptic terminals determine cell-intrinsic properties of neurons; however, the cellular mechanisms selectively controlling establishment and maintenance of neuronal compartments remain poorly understood. Here, we show that two giant Drosophila Ankyrin2 isoforms, Ank2-L and Ank2-XL, and the MAP1B homolog Futsch form a membrane-associated microtubule-organizing complex that determines axonal diameter, supports axonal transport, and provides independent control of synaptic dimensions and stability. Ank2-L controls microtubule and synaptic stability upstream of Ank2-XL that selectively controls microtubule organization. Synergistically with Futsch, Ank2-XL provides three-dimensional microtubule organization and is required to establish appropriate synaptic dimensions and release properties. In axons, the Ank2-XL/Futsch complex establishes evenly spaced, grid-like microtubule organization and determines axonal diameter in the absence of neurofilaments. Reduced microtubule spacing limits anterograde transport velocities of mitochondria and synaptic vesicles. Our data identify control of microtubule architecture as a central mechanism to selectively control neuronal dimensions, functional properties, and connectivity. Stephan et al. demonstrate that in Drosophila, two giant Ankyrin2 isoforms and Futsch/MAP1B form a membrane-associated MT-organizing complex essential for the control of synaptic dimensions, function, and stability. In axons, Ank2-XL and Futsch/MAP1B establish grid-like microtubule organization and determine the axonal diameter in the absence of neurofilaments.
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DOI：
10.1083/jcb.201305134
发表时间：
2014-01-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bulat V;Rast M;Pielage J
通讯作者：
Pielage J
影响因子：
9.8
作者：
Enneking EM;Kudumala SR;Moreno E;Stephan R;Boerner J;Godenschwege TA;Pielage J
通讯作者：
Pielage J
DOI：
10.1083/jcb.150.2.f63
发表时间：
2000-07-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goldstein LS;Gunawardena S
通讯作者：
Gunawardena S
影响因子：
64.5
作者：
Galiano MR;Jha S;Ho TS;Zhang C;Ogawa Y;Chang KJ;Stankewich MC;Mohler PJ;Rasband MN
通讯作者：
Rasband MN
影响因子：
--
作者：
FRIEDE, RL;SAMORAJS.T
通讯作者：
SAMORAJS.T