Changes in Holstein cow milk and serum proteins during intramammary infection with three different strains of Staphylococcus aureus.
Changes in Holstein cow milk and serum proteins during intramammary infection with three different strains of Staphylococcus aureus.
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DOI：
10.1186/1746-6148-7-51
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发表时间：
2011-09-01
影响因子：
2.6
通讯作者：
Karrow N
中科院分区：
文献类型：
--
作者：
Kim Y;Atalla H;Mallard B;Robert C;Karrow N
金黄色葡萄球菌是引起奶牛乳房炎的主要病原菌之一。奶牛乳房内感染沙门氏菌。由于病原体能够逃避先天防御机制，金黄色葡萄球菌通常是亚临床的，但这可能导致慢性感染。S.金黄色葡萄球菌被称为小菌落变种（SCV），表现出非典型的表型特征，导致持续性感染，并且比亲本菌株对抗生素的耐药性更强。因此，假设SCV的宿主免疫应答与其亲本或典型的S.金黄色。本研究采用免疫组化法检测了三株乳内沙门氏菌感染后局部和全身免疫蛋白的变化。金黄色葡萄球菌，包括天然存在的牛SCV菌株（SCV Heba 3231）。在乳房内感染三种沙门氏菌后，随着时间的推移，监测血清和去除酪蛋白的牛奶细胞因子水平（白细胞介素-8、干扰素-γ和转化生长因子-β1）以及血清触珠蛋白浓度。金黄色葡萄球菌菌株。此外，比较蛋白质组学被用来评估牛奶蛋白质组谱在急性和慢性阶段的S。金黄色葡萄球菌乳房内感染不同菌株感染后，奶牛血清中IL-8、IFN-γ和TGF-β1的反应不同。金黄色。观察到每种S.金黄色葡萄球菌攻毒组与对照组比较，差异无统计学意义（P> 0.05）。在去除酪蛋白的牛奶中，观察到宿主IFN-γ应答的菌株特异性差异，但诱导型IL-8和TGF-β1浓度在组间无差异。乳房内感染后的牛奶的蛋白质组学分析揭示了独特的宿主蛋白质表达谱，这取决于感染菌株以及感染阶段。值得注意的是，蛋白质，蛋白糖蛋白胨（CPP 3）的组分3，在S。金黄色葡萄球菌处理组，暗示它作为一个潜在的抗菌肽参与宿主防御对S。金黄色葡萄球菌乳房内感染奶牛乳房内感染沙门氏菌。金黄色葡萄球菌引起血清和乳汁免疫相关蛋白的上调，这些反应因感染菌株而异。
Staphylococcus aureus is one of the most prevalent pathogens to cause mastitis in dairy cattle. Intramammary infection of dairy cows with S. aureus is often subclinical, due to the pathogen's ability to evade the innate defense mechanisms, but this can lead to chronic infection. A sub-population of S. aureus, known as small colony variant (SCV), displays atypical phenotypic characteristics, causes persistent infections, and is more resistant to antibiotics than parent strains. Therefore, it was hypothesized that the host immune response will be different for SCV than its parental or typical strains of S. aureus. In this study, the local and systemic immune protein responses to intramammary infection with three strains of S. aureus, including a naturally occurring bovine SCV strain (SCV Heba3231), were characterized. Serum and casein-depleted milk cytokine levels (interleukin-8, interferon-γ, and transforming growth factor-β1), as well as serum haptoglobin concentrations were monitored over time after intramammary infection with each of the three S. aureus strains. Furthermore, comparative proteomics was used to evaluate milk proteome profiles during acute and chronic phases of S. aureus intramammary infection. Serum IL-8, IFN-γ, and TGF-β1 responses differed in dairy cows challenged with different strains of S. aureus. Changes in overall serum haptoglobin concentrations were observed for each S. aureus challenge group, but there were no significant differences observed between groups. In casein-depleted milk, strain-specific differences in the host IFN-γ response were observed, but inducible IL-8 and TGF-β1 concentrations were not different between groups. Proteomic analysis of the milk following intramammary infection revealed unique host protein expression profiles that were dependent on the infecting strain as well as phase of infection. Notably, the protein, component-3 of the proteose peptone (CPP3), was differentially expressed between the S. aureus treatment groups, implicating it as a potential antimicrobial peptide involved in host defense against S. aureus intramammary infection. Intramammary infection of dairy cattle with S. aureus causes an up-regulation of serum and milk immune-related proteins, and these responses vary depending on the infecting strain.
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影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
3.3
作者：
Atalla, Heba;Gyles, Carlton;Mallard, Bonnie
通讯作者：
Mallard, Bonnie
影响因子：
5.4
作者：
Birebent, B;Lorho, R;Semana, G
通讯作者：
Semana, G
DOI：
10.1128/cdli.11.3.463-472.2004
发表时间：
2004-05-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Bannerman, DD;Paape, MJ;Rainard, P
通讯作者：
Rainard, P
影响因子：
3.5
作者：
Almeida, RA;Matthews, KR;Oliver, SP
通讯作者：
Oliver, SP