Effect of activated carbon amendment on bacterial community structure and functions in a PAH impacted urban soil.
Effect of activated carbon amendment on bacterial community structure and functions in a PAH impacted urban soil.
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DOI：
10.1021/es2043905
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发表时间：
2012-05-01
影响因子：
11.4
通讯作者：
Werner, David
中科院分区：
文献类型：
--
作者：
Meynet, Paola;Hale, Sarah E.;Davenport, Russell J.;Cornelissen, Gerard;Breedveld, Gijs D.;Werner, David
我们收集了城市土壤样品的多环芳烃（PAHs）的影响，从sorbohydrin为基础的修复现场试验，以解决不必要的副作用的关注2%的粉末（PAC）或颗粒（GAC）活性炭修正剂对土壤微生物和污染物生物降解。三年后，总微生物细胞计数和呼吸速率最高的活性炭改良土壤。变性梯度凝胶电泳（DGGE）得到的优势细菌群落结构随着时间的推移比AC修正案的反应更强烈。DGGE条带测序显示，在所有土壤中存在与已知PAH降解剂（例如Rhodococcus jostii RHA-1）或已知含有PAH降解剂（例如Rhodococcus erythropolis）的类群最接近的类群。通过实时聚合酶链反应定量得到类似的双加氧酶基因拷贝数在未经修正，PAC，或GAC修正的土壤。PAH的可用性评估在批量测试中显示，最大的差异为75%，有和没有生物杀灭剂添加未修正的土壤，而最低的PAH的可用性整体上测得的PAC修正，活的土壤。我们的结论是，AC对土壤微生物学没有不利影响，AC修正土壤保留了生物降解多环芳烃的潜力，但生物降解去除有效污染物是最显着的未修正的土壤。
We collected urban soil samples impacted by polycyclic aromatic hydrocarbons (PAHs) from a sorbent-based remediation field trial to address concerns about unwanted side-effects of 2% powdered (PAC) or granular (GAC) activated carbon amendment on soil microbiology and pollutant biodegradation. After three years, total microbial cell counts and respiration rates were highest in the GAC amended soil. The predominant bacterial community structure derived from denaturing gradient gel electrophoresis (DGGE) shifted more strongly with time than in response to AC amendment. DGGE band sequencing revealed the presence of taxa with closest affiliations either to known PAH degraders, e.g. Rhodococcus jostii RHA-1, or taxa known to harbor PAH degraders, e.g. Rhodococcus erythropolis, in all soils. Quantification by real-time polymerase chain reaction yielded similar dioxygenases gene copy numbers in unamended, PAC-, or GAC-amended soil. PAH availability assessments in batch tests showed the greatest difference of 75% with and without biocide addition for unamended soil, while the lowest PAH availability overall was measured in PAC-amended, live soil. We conclude that AC had no detrimental effects on soil microbiology, AC-amended soils retained the potential to biodegrade PAHs, but the removal of available pollutants by biodegradation was most notable in unamended soil.
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影响因子：
4.4
作者：
Griffiths, RI;Whiteley, AS;Bailey, MJ
通讯作者：
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影响因子：
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