Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
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DOI：
10.1016/j.clinbiochem.2011.06.983
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发表时间：
2011-10
影响因子：
2.8
通讯作者：
Geluk A
中科院分区：
文献类型：
--
作者：
Corstjens PL;de Dood CJ;van der Ploeg-van Schip JJ;Wiesmeijer KC;Riuttamäki T;van Meijgaarden KE;Spencer JS;Tanke HJ;Ottenhoff TH;Geluk A
The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases. A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients. Detection of IL-10 below the targeted level of 100 pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R2 value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated. The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
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影响因子：
9.4
作者：
Corstjens, Paul L. A. M.;van Lieshout, Lisette;van Dam, Govert J.
通讯作者：
van Dam, Govert J.
影响因子：
2.8
作者：
Corstjens, Paul L. A. M.;Zuiderwijk, Michel;Geluk, Annemiek
通讯作者：
Geluk, Annemiek
DOI：
10.1196/annals.1384.021
发表时间：
2007-01-01
期刊：
ORAL-BASED DIAGNOSTICS
影响因子：
--
作者：
Mokkapati, Vijaya K.;Niedbala, R. Sam;Corstjens, Paul L. A. M.
通讯作者：
Corstjens, Paul L. A. M.
影响因子：
3.1
作者：
CHO, SN;YANAGIHARA, DL;BRENNAN, PJ
通讯作者：
BRENNAN, PJ
影响因子：
5.5
作者：
Verreck, Frank A. W.;de Boer, Tjitske;Ottenhoff, Tom H. M.
通讯作者：
Ottenhoff, Tom H. M.